摘要
目的研究自腹泻病例和家用冰箱中分离的小肠结肠炎耶尔森菌(Yersinia enterocolitica,Ye)的病原学特征,为科学防控Ye的污染提供依据。方法采集2017年北京市顺义区2家哨点医院肠道门诊腹泻患者病例粪便标本和顺义区83户家用冰箱中的涂抹拭子样品,对分离的Ye进行毒力基因、脉冲场凝胶电泳(PFGE)分子分型检测和耐药试验。结果腹泻病例粪便标本中Ye阳性率为0.27%(1/372),家用冰箱中Ye阳性率为6.02%(5/83);冰箱分离株仅携带ystB基因,腹泻病例分离株携带ail、ystA、virF、yadA基因;腹泻病例和冰箱分离株PFGE带型亲缘关系较远。全部Ye对氨苄西林、阿莫西林/克拉维酸、头孢唑林均耐药,腹泻病例分离株对萘啶酸耐药。结论 Ye在北京市顺义区腹泻病例中流行强度不高,家用冰箱受Ye污染较为严重,腹泻病例和冰箱中分离的Ye病原学特征具有一定差异。
Objective To study the etiological characteristics of Yersinia enterocolitica(Ye)isolated from diarrheal patients and household refrigerators,and to provide basis for scientific prevention and control of Ye.Methods Stool samples were collected from diarrhea patients from two outpatients in two hospitals in Shunyi District,Beijing in 2017.Smear samples were collected from 83 household refrigerators in Shunyi District.Ye was isolated and cultured,and the isolated stains of Ye were tested for toxin genes,pulsed field gel electrophoresis(PFGE)molecular typing and drug resistance tests.Results The positive rate of Ye was 0.27%(1/372)in stool samples of diarrhea patients and 6.02%(5/83)in household refrigerators.The strains isolated from refrigerators only carried the ystB gene and the strains isolated from diarrhea patients carried ail,ystA,virF and yadA genes.Six Ye strains showed 4 PFGE patterns with a low similarity.All Ye strains were resistant to ampicillin,amoxicillin/clavulanic acid and cefazolin,and the Ye strain isolated from diarrhea patient was resistant to nalidixic acid.Conclusion The prevalence of Ye was not high in the patients of diarrhea in Shunyi District of Beijing.Ye contaminationin household refrigerators was more serious.Ye pathogenic characteristics in environment and diarrhea patient were different.
作者
张彦春
张爽
杨杰
王苗
王园园
李颖
ZHANG Yanchun;ZHANG Shuang;YANG Jie;WANG Miao;WANG Yuanyuan;LI Ying(Shunyi District Center for Disease Control and Prevention,Beijing 101300,China)
出处
《中国食品卫生杂志》
北大核心
2018年第6期582-585,共4页
Chinese Journal of Food Hygiene
作者简介
张彦春,女,主管检验师,研究方向为微生物,E—mail:1120714627@qq.com;通信作者:李颖,男,主管检验师,研究方向为病原微生物,E-mail:liying19830805@126.com.
