摘要
为了探讨红花注射液各生产工艺环节对红花注射液品质的影响,该研究以测定活化部分凝血活酶时间(APTT)和二磷酸腺苷(ADP)诱导的体外抑制血小板聚集率为指标,评价了红花注射液生产过程中的提取、浓缩、2次醇沉、水沉、2次灭菌等关键环节中间体的生物活性,并运用HPLC测定了主要化学成分的含量。结果表明,随着红花注射液的制备工艺流程进展,各中间体的体外抑制血小板聚集率逐渐降低,延长APTT活性变化趋势为先降低然后又升高。此外,羟基红花黄色素A(HSYA)的含量逐渐降低,对羟基肉桂酸的含量升高,并且产生了新的化学成分对羟基苯甲醛。以上结果说明在红花注射液生产制备关键环节中,灭菌环节对红花注射液的生物活性和HSYA的含量影响较大。
In order to investigate the effect of various production processes on the quality of Safflower Injection,the biological activities of the intermediates were evaluated by measuring activated partial thromboplastin time( APTT) and adenosine diphosphate( ADP) induced platelet aggregation in vitro. Intermediates were produced by key processes,such as extraction,concentration,twice alcohol precipitation,water sedimentation and two sterilizations during the production of Safflower Injection. The content of main chemical components in intermediates was determined by HPLC. The results showed that with the advance of the preparation process of Safflower Injection,the inhibition of ADP-induced platelet aggregation rate of each intermediate decreased gradually,and the trend of extending APTT activity decreased first and then increased. Meanwhile,the content of hydroxy safflor yellow A( HSYA) was gradually lowered,the content of p-hydroxy-cinnamic acid was increased,and new chemical component p-hydroxybenzaldehyde was produced. In conclusion,sterilization played a key role in the biological activity and HSYA content of Safflower injection.
作者
梁萌萌
李石飞
王凯红
张立伟
LIANG Meng-meng;LI Shi-fei;WANG Kai-hong;ZHANG Li-wei(Key Laboratory of Chemical Biology and Molecular Engineering under Ministry of Education,Institute of Molecular Science, Shanxi University;Modern Research Center for Traditional Chinese Medicine,Taiyuan 030006,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2018年第24期4850-4854,共5页
China Journal of Chinese Materia Medica
基金
国家中药标准化项目(ZYBZH-C-JIN-44)
山西省重点研发计划项目(201603D3113008)
关键词
红花注射液
羟基红花黄色素A
对羟基苯甲醛
对羟基肉桂酸
血小板聚集
凝血活酶时间
Safflower Injection
hydroxy safflor yellow A
p-hydroxybenzaldehyde
p-hydroxy-cinnamic acid
platelet aggregation
activated partial thromboplastin time(APTT)
作者简介
梁萌萌,硕士研究生,研究方向为天然药物化学,E-mail:1079667102@qq.com;通信作者:张立伟,教授,研究方向为天然药物化学,E-mail:lwzhang@sxu.edu.cn.
