摘要
为建立小黄姜人工种子的制作方法,以贵州地方品种六盘水小黄姜姜芽为外植体,通过组织培养的方法获得丛生芽,以丛生芽作为小黄姜人工种子的包埋繁殖体即人工种胚,研究不同人工种皮基质、胚乳组成、络合时间、贮藏条件以及萌发基质等因素对小黄姜人工种子萌发与成苗的影响。结果表明,人工种皮基质为2.0%CaCl2+2.0%壳聚糖+4.0%海藻酸钠、胚乳成分为MS液体培养基+0.1 mg/L青霉素+0.1%苯甲酸钠+2.0%蔗糖+0.3%活性炭+4.0 mg/L 6-BA+1.0 mg/L NAA时,人工种子在无菌环境中的萌发率和成苗率最高,分别达到86.67%、83.33%。通过贮藏条件的研究,发现4℃条件下贮藏10 d后其萌发率及成苗率分别为63.33%、60.00%。此外,利用不同萌发基质对人工种子进行萌发试验,结果表明播种于灭菌复合基质(营养土∶蛭石=2∶1)以及附加营养液(MS+0.1 mg/L 6-BA+0.1 mg/L NAA)时,人工种子的萌发率和成苗率最高,分别为61.67%、58.33%,以丛生芽作为人工种胚的小黄姜人工种子制作技术具有生产应用前景。
In order to establish the manufacture method for artificial seeds of small yellow ginger, using Guizhou local variety, Liupanshui small yellow ginger sprout as explants, clustered buds were obtained by tissue culture.Taking clustered buds as embedding propagule(artificial embryo) of small yellow ginger artificial seed, this study researched the effect of different artificial seed-coat matrixes, components of endosperm, complex time, storage conditions and germinative substrates on the germination and seedling of small yellow ginger seeds. The result showed that when 4.0% sodium alginate+2.0% CaCl2+2.0% chitosan was served as artificial seed-coat matrix, and MS liquid culture medium+0.1 mg/L penicillin+0.1% sodium benzoate+2.0% sucrose+0.3% activated carbon+4.0 mg/L6-BA+1.0 mg/L NAA was served as endosperm components, the artificial seeds had the highest germination rate and seedling rate in a sterile environment, which were 86.67% and 83.33%, respectively. Through the studies onsto rage conditions, it was found that after storing at 4℃ for 10 d, the germination rate and seedling rate were63.33% and 60.00%, respectively. In addition, sprouting experiment for artificial seeds with different germinative substrates was carried out, and the results showed that when sowing on the sterile composite substrate(nutrient soil∶vermiculite=2∶1) and spraying the nutrient solution(MS+0.1 mg/L 6-BA+0.1 mg/L NAA), the germination rate and seedling rate of artificial seeds were the highest, which were 61.67% and 58.33%, respectively. Artificial seed manufacture method of small yellow ginger with clustered buds as artificial embryos might have application prospects.
作者
李晋华
赵德刚
赵懿琛
Li Jinhua;Zhao Degang;Zhao Yichen(The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education),Guizhou Key Lab of Ago-Bioengineering,Institute of Ago-Bioengineering,College of Life Sciences,Guizhou University,Guiyang,550025;The 2011 Collaboration Innovation Center for Mountain Ecology and Agro-Bioengineering in Guizhou Province,Guiyang,550025;College of Tea Sciences,Guizhou University,Guiyang,550025;Guizhou Academy of Agricultural Sciences,Guiyang,550006)
出处
《分子植物育种》
CAS
CSCD
北大核心
2018年第24期8148-8154,共7页
Molecular Plant Breeding
基金
贵州省科技计划项目(黔科合LH字[2014]7678)资助.
关键词
小黄姜
人工种子
丛生芽
Zingiber offiinale Rosceoe
Artificial seed
Clustered bud
作者简介
通讯作者:赵德刚,dgzhao@gzu.edu.cn;通讯作者:赵懿琛,yczhao@gzu.edu.cn.
