摘要
目的:比较和评价培养北虫草子实体与野生冬虫夏草子实体的特征性化学活性成分。方法:运用现代化学分析技术,对人工低温培养的北虫草的子实体与野生冬虫夏草的主要化学成分虫草酸、虫草多糖、腺苷、超氧化物岐化酶、氨基酸组分等进行了比较分析。结果:培养北虫草子实体中虫草素含量(12.700±0.544) g/kg,为野生冬虫夏草子实体的302倍,腺苷含量为(0.037%±0.010)%,为野生冬虫夏草子实体的1.42倍,虫草多糖(5.680%±1.500%)高于野生冬虫夏草46%。培养北虫草子实体中可溶性蛋白总量(34.200%±8.090%)为野生虫草的1.31倍,且SOD活性达1616 U/g,但野生冬虫夏草中SOD活性极低。氨基酸分析结果显示,培养北虫草子实体中具有17种必需氨基酸,除甘氨酸和甲硫氨酸外,其它氨基酸含量均高于野生冬虫夏草子实体。结论:人工培养北虫草子实体,具有高含量活性成分,可以深度开发替代野生冬虫夏草作为药用材料和食品保健开发。
Objective: To characterize and evaluate main active chemical components of cultured Cordyceps militaris and Cordyceps sinensis. Methods: The fruiting body of Cordyceps acid, Cordyceps polysaccharide,adenosine, superoxide dismutase, amino acid were analyzed in cultured Cordyceps militaris and Cordyceps sinensis, with modern analysis technology. Results: The cordycepin content in fruiting body of cultured C.militaris was(12.700±0.544) g/kg, which was 302 folds of natural fruiting body of C. sinensis. Adenosine content of cultured C. militar was(0.037±0.010)%, which was 1.42 fold that in natural fruiting body of C. sinensis and polysaccharide content(5.680% ±1.500%) was also increased 46% in C. militaris. Moreover, the total soluble protein in fruiting body of cultured C. militaris(34.200%±8.090%) was 1.31 times that of natural fruiting body of C. sinensis and contained higher active SOD(1616 U/g), however, SOD activity is quite low in C. sinensis.Amino acid analysis data showed that the fruiting body of cultured C. militaris has 17 types of amino acids,except glycine and methionine, the other amino acids content was higher than that of wild C. sinensis.Conclusion: The fruiting body of culture C. militaris has the higher active components and could replace C.sinensis to be exploited further as the medicinal material or food healthy product.
作者
王环
王爱珍
程大志
沈建伟
陈世龙
周党卫
WANG Huan;WANG Ai-zhen;CHENG Da-zhi;SHEN Jian-wei;CHEN Shi-long;ZHOU Dang-wei(Key Lab of Adaptation and Evolution of Plateau Biota,Northwest Plateau Institute of Biology,Chinese Academy of Sciences,Xi'ning Qinghai 810008,China;School of Life Science,Qinghai University,Xi'ning Qinghai 810016,China;Qinghai Shifeng Life Sci&Tec Company,Xi'ning Qinghai 810003,China)
出处
《中医药导报》
2019年第1期79-82,共4页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
中科院知识创新项目(KSCX2-SW-106)
青海省基础研究项目资助(2009-N-305
2017-ZJ-702)
关键词
北虫草
子实体
冬虫夏草
化学成分
Cordyceps militaris
cordyceps sinesis
Fruiting body
chemical components
作者简介
通讯作者:周党卫,E-mail:dangweizhou@sina.com.
