摘要
土壤细菌群落组成在森林生态系统中有着至关重要的作用。本文采用Illumina Mi Seq高通量测序技术对土壤细菌16S rDNA的V4～V5可变区进行测序,探讨了南亚热带地区5种典型乡土树种人工纯林(红椎(Castanopsis hystrix)、米老排(Mytilaria laosensis)、火力楠(Michelia macclurei)、山白兰(Paramichelia bailonii)和马尾松(Pinus massoniana))和外来速生桉树(Eucalyptus)人工林土壤表层(0～20 cm)与底层(20～40 cm)的细菌群落组成的差异性。结果表明:6种林分土壤表层与底层共有已定义细菌类群20门34纲48目82科131属110种,其中酸杆菌门(Acidobacteria)和变形菌门(Proteobacteria)为绝对优势菌门。在门、纲、目分类水平上均表现出桉树林土壤细菌群落组成与其他5种乡土人工林存在明显差异,并建议在目分类水平上可较好的表征南亚热带地区人工纯林土壤细菌群落组成间的差异性。
Soil bacteria have important ecological functions in the forest ecosystem. The variable regions (V4 ~ V5) of 16S rDNA gene of soil bacteria were examined by using Illumina MiSeq high-throughput sequencing in order to investigate the changes of bacterial community composition in the topsoil (0 ~ 20 cm) and subsoil (20 ~ 40 cm) from five typical native monoculture plantations (Castanopsis hystrix, Mytilaria laosensis, Michelia macclurei, Paramichelia bailonii, and Pinus massoniana) and one extraneous fast-growing species (Eucalyptus) in south subtropical China. The results showed that both soil layers contained 21 phyla, 34 classes, 47 orders, 82 families, and 131 genera of classified bacteria, and Acidobacteria and Proteobacteria were the absolute dominant phyla. At the levels of phylum, class, and order, the soil bacterial community composition of Eucalyptus showed significant differences among five native monoculture plantations. The order taxonomic level would be suggested to well characterize the differences in soil bacterial community composition among various monoculture plantations in south subtropical China.
作者
彭雯
谭玲
明安刚
何友均
覃林
PENG Wen;TAN Ling;MING An-gang;HE You-jun;QIN Lin(College of Forestry, Guangxi University, Nanning 530004, China;Experimental Centre of Tropical Forestry, Chinese Academy of Forestry, Pingxiang 532600, China;Research Institute of Forestry Policy and Information, Chinese Academy of Forestry, Beijing 100091,China;Guangxi Key Laboratory of Forest Ecology and Conservation, College of Forestry, Guangxi University, Nanning 530004, China)
出处
《土壤通报》
CAS
北大核心
2018年第6期1361-1369,共9页
Chinese Journal of Soil Science
基金
国家自然科学基金项目(31560109)资助
关键词
土壤细菌
群落组成
人工纯林
高通量测序技术
南亚热带
Soil bacteria
Community composition
Monoculture plantation
Illumina MiSeq sequencing
South subtropical China.
作者简介
彭雯(1992一),女,湖北武汉人,硕士。主要从事森林生态研究。E-mail:619639854@qq.com;通讯作者:覃林,E—mail:nilniq@gxu.edu.cn
