摘要
目的:探讨再生胶囊对再生障碍性贫血大鼠外周血CD4+、CD25+调节性T细胞(Treg)、核转录因子Foxp3表达及细胞因子IL-10、TGF-β1的影响。方法:将80只SD大鼠随机分成空白组、模型组、阳性对照组和再生胶囊组,每组20只。除空白组外,其余各组大鼠予60Co-γ联合腹腔注射环磷酰胺和氯霉素建立再生障碍性贫血大鼠模型。于造模第7天再生胶囊组给予再生胶囊[0.216 g/(kg·d)]灌胃,模型组和空白组予等体积的生理盐水灌胃,阳性对照组予环孢素[3 mg/(kg·d)]灌胃,各组均干预30 d。检测大鼠外周血白细胞数(WBC)、网织红细胞数(Ret)、血红蛋白数(Hb)及血小板数(PLT),骨髓有核细胞数(粒系比例、红系比例、淋巴细胞比例、巨核细胞数),细胞因子IL-10和TGF-β1浓度,Treg细胞比例和单个核细胞Foxp3 mRNA表达量。结果:与空白组比较,模型组大鼠外周血WBC、Ret、Hb、PLT、骨髓粒系比例、红系比例、巨核细胞数、IL-10浓度、TGF-β1浓度、Treg细胞比例、Foxp3 mRNA表达量降低,淋巴细胞比例升高(P<0.05)。与模型组比较,再生胶囊组和阳性对照组大鼠外周血WBC、Ret、Hb、PLT、骨髓粒系比例、红系比例、巨核细胞数、IL-10浓度、TGF-β1浓度、Treg细胞比例、Foxp3 mRNA表达量升高,淋巴细胞比例下降(P<0.05)。与阳性对照组比较,再生胶囊组大鼠外周血WBC、Ret、Hb、PLT、骨髓粒系比例、红系比例、巨核细胞数、IL-10浓度、TGF-β1浓度、Treg细胞比例、Foxp3mRNA表达量升高,淋巴细胞比例下降(P<0.05)。结论:再生胶囊能通过促使再生障碍性贫血大鼠外周血Treg细胞数和FOXP3表达量增多,产生抑制性细胞因子IL-10和TGF-β1,恢复自身免疫耐受平衡,改善骨髓造血衰竭。
Objective:To explore the effect of Zaisheng capsule on CD4^+CD25^+-regulatory T cell(Treg),transcription factor Foxp3 mRNA and cytokines IL10/TGF-β1 in the peripheral blood of aplastic anemia(AA)rats.Methods:80 rats were randomly divided into the blank control group,the model group,the positive control group and Zaisheng capsule group,20 in each group.In addition to the blank control group,the other groups of rats were treated with60 Co-γcombined via intraperitoneal injection of cyclophosphamide and chloramphenicol to establish AA rat model.At the seventh day of modeling,Zaisheng capsule group of AA rat were intragastric administrated with the decoction at the dose of 0.216 g/(kg·d),separately;the model group and the normal rat group were given an equal volume of saline,and the positive control group was treated with cyclosporine of 3 mg/(kg·d).All groups were treated for 30 days.The white blood cell count(WBC),reticolociti(Ret),hemoglobin counts(Hb),platelet count(PLT),nucleated cells in bone marrow(the portion of granule count,the portion of red cells,the portion of lymphocytes,megakaryocytes) were measured.Cytokines IL-10,TGF-β1,proportion of Treg cell and the expression of mRNA Foxp3 in the peripheral blood mononuclear cells of each group were detected.Results:Compared with the blank controller group,the WBC counting,Ret,Hb and PLT level,the portion of bone marrow granule,red cells and megakaryocytes,cytokines IL-10,TGF-β1,the proportion of Treg cells,the expression of Foxp3 mRNA of the peripheral blood in the model group decreased significantly,and the portion of lymphocytes increased significantly(P<0.05).Compared with the model group,the WBC counting,Ret,Hb and PLT level,the portion of bone marrow granule,red cells and megakaryocytes,cytokines IL-10,TGF-β1,the proportion of Treg cells,the expression of Foxp3 mRNA of the peripheral blood in the Zaisheng capsule group and positive group increased significantly,and the portion of lymphocytes decreased significantly(P <0.05).Compared with the positive group,the WBC counting,Ret,Hb and PLT level,the portion of bone marrow granule,red cells and megakaryocytes,cytokines IL-10,TGF-β1,the proportion of Treg cells,the expression of Foxp3 mRNA of the peripheral blood in the Zaisheng capsule group increased significantly,and the portion of lymphocytes decreased significantly(P<0.05).Conclusions:Zaisheng capsule can promote the proportion of Treg cell and expression of Foxp3 mRNA of peripheral blood in AA rat,produce inhibitory cytokines IL-10 and TGF-β1,restore the balance of autoimmune tolerance,and improve the hematopoietic failure of bone marrow.
作者
聂甜
江劲波
王跃
杨琳
郝敬全
黄蓉
刘凯
NIE Tian;JIANG Jin-bo;WANG Yue;YANG Lin;HAO Jing-quan;HUANG Rong;LIU Kai(The First Affiliated Hospital of Hu'nan University of Chinese Medicine,Changsha Hu'nan 410007,China)
出处
《中医药导报》
2018年第24期34-38,共5页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
湖南省科技厅一般项目(2013SK3102)
作者简介
通讯作者:江劲波,E-mail:568098977@qq.com
