摘要
一个自组装的金属有机框架Dy-NH2-BDC微晶可以采用DMF和CH2Cl2洗涤配合物Dy-NH2-BDC得到.经叶酸(FA)和双端羧基聚乙二醇(HOOC-PEG-COOH)修饰,可以得到Dy-NH2-FA和Dy-PEGCOOH样品.Dy-NH2-FA和Dy-PEG-COOH的结构经红外(IR)确证.热重分析(TGA)用来表征DOX(阿霉素,用于治疗许多常见癌症的蒽环类化学治疗剂)的装载情况.药物释放实验表明,DOX/Dy-NH2-FA样品中的DOX在酸性肿瘤环境中比正常体液环境(pH=7.4)释放快.DOX/Dy-PEG-COOH样品(比例20∶10和20∶20)在PBS缓冲液(pH=5.8, 0.1 mol/L)中释放144 h的荧光强度分别为77.6和196.7,接近于DOX在0.002和0.008mg/mL时的强度,均比DOX/Dy-NH2-FA样品(比例20∶10和20∶20)的荧光强度高.结果表明Dy-PEG-COOH样品的药物传递能力优于叶酸修饰系统,但并未得到良好的释放曲线.考虑到聚乙二醇的较大分子量,FA修饰系统与PEG修饰系统有诸多不同.
A self-assembled functionalized metal-organic frameworks (MOFs), Dy-NH2-BDC microcrystalline could be prepared by washing complex Dy-NH2-BDC with DMF and CH2Cl2. Via the modification of folic acid (FA) and dicarboxylic acid-terminated polyethylene glycol (HOOC-PEG-COOH), Dy- NH2-FA and Dy-PEG-COOH samples could be obtained. IR was used for structural confirmation of Dy- NH2-FA and Dy-PEG-COOH samples. Doxorubicin (DOX) is a member of the anthracycline class of chemotherapeutic agents that are used for the treatment of many common human cancers. TGA was used for determination of DOX loading. Drug-release experiments implied that DOX from the DOX/Dy-NH2- FA samples could be released faster at acidic tumor tissue than at normal body fluid (pH=7.4). The intensity of the DOX/Dy-PEG-COOH samples (20∶10 and 20∶20) at 593 nm (λex=480 nm) against PBS buffer solution (pH=5.8, 0.1 mol/L) were 77.6 and 196.7 at 144 h, close to the intensity of DOX at the concentration of 0.002 and 0.008 mg/mL, which were higher than those of the DOX/Dy-NH2-FA samples (20∶ 10 and 20∶20). The results showed that the drug delivery capacity of Dy-PEG-COOH samples was better than FA loaded system, but no good release curves were obtained. Taking into account the large molecular weight of polyethylene glycol, FA loaded system was different from PEG loaded system.
作者
杨琳燕
臧莲娜
秦铭杉
刘泓
Yang Linyan;Zang Lianna;Qin Mingshan;Liu Hong(College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China)
出处
《南开大学学报（自然科学版）》
CAS
CSCD
北大核心
2018年第6期79-86,共8页
Acta Scientiarum Naturalium Universitatis Nankaiensis
基金
Supported by the Research Project of Tianjin Education Commission(2017KJ190)
关键词
氨基功能化
稀土配合物
修饰
药物传递系统
amino-functionalized
rare earth complexes
modification
drug delivery system
作者简介
Yang Linyan(1982-), female, native place: Hebei Jinzhou, lecturer, direction of research: Nanoscale drug carrier. E-mail:y_linyan@163.com
