摘要
为了探究温度胁迫下刺芹侧耳中过氧化氢酶和铜锌超氧化物歧化酶基因的作用,本研究通过RT-PCR方法对刺芹侧耳转录组数据库检索得到的过氧化氢酶(CAT)和铜锌超氧化物歧化酶(Cu/Zn-SOD)基因进行克隆,并利用荧光定量PCR分析在高低温胁迫下CAT和Cu/Zn-SOD基因的表达量,以探讨刺芹侧耳中这两种保护酶基因在高、低温胁迫下的响应特征。结果表明,克隆得到的CAT和Cu/Zn-SOD基因开放阅读框长度分别为1 581bp和582bp,分别编码526个和193个氨基酸,分子量分别为59.6kDa、19.62kDa,等电点分别为6.35、6.31。在高温(35℃)和低温(4℃)两组处理中,CAT和Cu/Zn-SOD基因的表达量及酶活均在处理48h时达到最高且与对照组呈现显著差异,说明两种基因在刺芹侧耳抵御高低温胁迫的过程中具有重要作用。本研究结果初步研究了温度胁迫下刺芹侧耳中CAT与Cu/Zn-SOD基因的生物学功能,为进一步解析刺芹侧耳抵抗温度胁迫的机理奠定基础。
The effects of catalase and copper-zinc superoxide dismutase genes in Pleurotus eryngii under temperature stress were investigated.The catalase(CAT)and copper-zinc superoxide dismutase(Cu/Zn-SOD)genes were cloned by RT-PCR and their expression levels under high and low temperature stress were analyzed by real-time PCR.The results showed that the open reading frames of CAT and Cu/Zn-SOD genes were 1 581bp and 582bp,encoding 526 and 193 amino acids,respectively.The molecular weight of amino acids were 59.6kDa and 19.62kDa and the isoelectric points were 6.35 and 6.31,respectively.Under high temperature(35℃)and low temperature(4℃)treatment,the expression levels and enzyme activities of CAT and Cu/Zn-SOD genes reached the highest in 48h and both of them showed significant difference in comparison with untreated group,indicating CAT and Cu/Zn-SOD genes played an important role in the process of resisting temperature stress.
作者
闫苗
陈利丁
艾柳英
李帆
刘云超
董皓
孙淑静
YAN Miao;CHEN Li-Ding;AI Liu-Ying;LI Fan;LIU Yun-Chao;DONG Hao;SUN Shu-Jing(College of Lile Sciences,Fujian Agriculture and Forestry University,Fuzhou, Fujian 350002,China;Gutian Research Institute of Edible Fungi,Fujian Agriculture and Forestry University Ningde, Fujian 35220,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2018年第12期1671-1679,共9页
Mycosystema
基金
福建省科技厅星火计划项目(2016S0041)
校科技创新专项基金(KFA17576A)~~
关键词
刺芹侧耳
基因克隆
序列分析
基因表达
酶活分析
Pleurotus eryngii
gene cloning
sequence analysis
gene expression
enzyme activity analysis
作者简介
通讯作者:孙淑静,E-mail:shjsun2004@126.com
