摘要
目的:比较全国不同产地野生桑黄中总黄酮的含量。方法:采用70%乙醇回流提取各产地的野生桑黄总黄酮,利用AB-8型大孔吸附树脂对总黄酮进行纯化,所得总黄酮供试品经紫外分光光度法测定总黄酮含量,以芦丁为对照品,波长为507 nm。结果:吉林长白山、山东夏津、西藏昌都、云南临沧、安徽金寨、四川甘孜6个产地的野生桑黄中总黄酮含量分别为62.22、55.84、60.08、37.64、48.63和39.90 mg/g。结论:所建立的方法能准确快速地对样品中的总黄酮进行测定;产地不同,野生桑黄的总黄酮含量存在差异。
Objective: To compare the content of total flavonoids in wild phellinus igniarius from different producing areas. Methods:Used 70% ethanol to reflux and extract total flavonoids from wild phellinus igniarius from different places,and purified total flavonoids by using AB-8 macroporous resin,and determined the content of total flavonoids by UV spectrophotometry. Rutin was used as the reference substance with a wave length of 507 nm. Results:The content of total flavonoids in wild phellinus igniarius from Changbai Mountain of Jilin Province,Xiajin of Shandong Province,Changdu of Tibet,Lincang of Yunnan Province,Jinzhai of Anhui Province and Ganzi of Sichuan Province was 62.22,55.84, 60.08,37.64,48.63 and 39.90 mg/g,respectively. Conclusion:The established method can determine the total flavonoids in samples accurately and quickly. The content of total flavonoids in wild phellinus igniarius was different in different producing areas.
出处
《新中医》
CAS
2018年第12期28-30,共3页
New Chinese Medicine
关键词
野生桑黄
总黄酮
含量比较
不同产地
大孔吸附树脂
紫外分光光度法
Wild phellinus igniarius
Total flavonoids
Comparison of content
Different producing areas
Macroporous resin
UV spectrophotometry
作者简介
胡碧辉(1975-),男,副主任中药师,研究方向:中药鉴定与成分分析。
