摘要
目的:探讨超滤管截留得到的大分子量灵芝多糖(ultrafiltered macromolecular Ganoderma lucidum polysaccharide,UM-GLP)对非小细胞肺癌细胞增殖、迁移和侵袭能力的抑制作用。方法:以非小细胞肺癌细胞A549和NCI-H1299作为研究对象,并以空白培养基和紫杉醇(PTX)处理分别作为空白对照和阳性对照。采用MTT法检测UM-GLP对A549和NCI-H1299细胞增殖的抑制作用,划痕实验检测UM-GLP对A549和NCIH1299细胞迁移能力的抑制作用,Transwell实验检测UM-GLP对A549和NCI-H1299细胞侵袭能力的抑制作用。结果:MTT实验结果显示,随着UM-GLP和PTX药物浓度的增加,细胞增殖抑制率明显增加,UM-GLP浓度在0.5~2.0 mg/ml时,与PTX浓度在25~100 nmol/L的抑制效果相仿。A549和NCI-H1299细胞划痕实验结果显示,与空白对照组比较,UM-GLP组和PTX组的迁移距离均明显缩短(P<0.05)。Transwell实验结果显示,UM-GLP(2 mg/ml)处理后,非小细胞肺癌细胞侵袭数量显著低于空白对照组(P<0.05);并且UM-GLP对A549细胞侵袭能力的抑制作用与紫杉醇组(0.1μmol/L)相当,对NCI-H1299细胞侵袭能力的抑制作用小于紫杉醇组(0.1μmol/L)(P<0.05)。结论:大分子量灵芝多糖可有效抑制非小细胞肺癌细胞A549和NCI-H1299的增殖、迁移和侵袭能力。
Objective: To investigate the inhibitory effects of ultrafiltered macromolecular Ganoderma lucidum polysaccharide ( UMGLP)on the proliferation,migration and invasion of non-small cell lung cancer cells ( NSCLC) . Methods: The non-small cell lungcancer cell lines A549 and NCI-H1299 were used as the study subjects. Blank culture medium and paclitaxel ( PTX) treatment were usedas the blank control and positive control,respectively. The inhibitory effect of UM-GLP on the proliferation of A549 cells and NCI-H1299cells was examined by MTT assay. The inhibitory effect of UM-GLP on the migration of A549 cells and NCI-H1299 cells was detected byscratch test. The inhibitory effect of UM-GLP on the invasion of A549 cells and NCI-H1299 cells was evaluated by Transwell assay.Results: The MTT results showed that,with the increase of UM-GLP and PTX concentration,the inhibition rate of cell proliferation wasincreased significantly. When the concentration of UM-GLP was at 0.5-2.0 mg /ml,the inhibition effect was similar to that of PTX at 25-100 nmol /L. In the scratch test,compared with the blank control group,the cell migration distances of A549 cells and NCI-H1299 cellswere significantly shortened in the UM-GLP group and PTX group ( P<0.05) . Transwell assay indicated that after treatment with UM-GLP( 2 mg /ml) ,the number of invaded NSCLC was significantly less than that in the blank control group ( P<0.05) ,the inhibitory effect ofUM-GLP on A549 cell invasion was comparable to that of the paclitaxel ( 0.1 μmol /L) ,and the inhibitory effect of UM-GLP on NCI-H1299cell invasion was weaker than that of paclitaxel ( 0.1 μmol /L) ( P<0.05) . Conclusion: UM-GLP can effectively inhibit the proliferation,migration and invasion of NSCLC A549 cells and NCI-H1299 cells.
作者
肖小龙
莫观
廉云
刘运蕾
李龙飞
XIAO Xiaolong;MO Guan;LIAN Yun;LIU Yunlei;LI Longfei(Wolimei Life Science Laboratory ( Shenzhen) Co.,Ltd.,Shenzhen 518107,China;School of Medicine,Southwest Medical University,Luzhou 646000,China)
出处
《上海中医药大学学报》
CAS
2018年第6期56-60,共5页
Academic Journal of Shanghai University of Traditional Chinese Medicine
基金
国家自然科学基金青年基金项目(81603176)
关键词
灵芝多糖
超滤管离心
非小细胞肺癌细胞
增殖
迁移
侵袭
Ganoderma lucidum polysaccharide
ultrafiltration centrifugation
non-small cell lung cancer cell
proliferation
migration
invasion
作者简介
肖小龙,男,硕士,工程师,主要从事细胞生物工程研究;通信作者:李龙飞,副研究员;E-mail: lee130428@163.com
