摘要
目的对成都地区驻地部队暴发55型腺病毒进行病原分离、全基因序列及和遗传进化特点等分子流行病学分析,为揭示55型腺病毒的进化起源及制订疾病防控策略提供参考数据。方法采集55型腺病毒感染者咽拭子标本,采用人喉癌上皮细胞HEp-2分离病毒株。利用分片段PCR和测序获取毒株的全基因序列,检索并下载GenBank数据库中55型腺病毒全基因序列,采用生物信息学分析软件-MEGA6. 06进行序列比对和系统发育进化树构建。结果从成都周边驻地部队3起感染疫情中分离出55型腺病毒5株,分别为24-1株、24-2株、1804001株、1806003株、DYN株。采用12片段分段PCR和测序获得了5株病毒全基因序列,GenBank登录号分别为MH256653～MH256657,5株病毒之间序列差异较小,仅有16处碱基变异。与我国报道的第一株55型腺病毒QS-DLL株和重庆株(CQ-2903)序列相比,有30处碱基变异。根据系统发育进化树分析结果,新分离的5株HAdV-55毒株均与QS-DLL株相近,与我们之前分离的西藏株(LS89/Tibet/2016)、云南株(KM03/YN/2016)等进化距离相对较远。结论本研究分析发现2018年成都周边驻地部队流行的55型腺病毒为QS-DLL类似株或重庆分离株CQ-2903株的突变株。
Objective To conduct a molecular epidemiological analysis of 3human adenovirus type 55(HAdV-55)outbreaks in the year 2018in military forces in Chengdu area and obtain epidemiological evidence to identify the evolutionary origin of HAdV-55for formulating disease prevention and control strategies.Methods Throat swab specimens from HAdV-55-infected individuals were collected for virus isolation via HEp-2cells.The full-length DNA sequences of the isolated HAdV-55strains were obtained with segmented PCR and sequencing.The whole genome sequences of HAdV-55retrieved from the GenBank database were used for sequence alignment and phylogenetic tree construction using bioinformatics analysis software MEGA6.05.Results Five strains of HAdV-55,namely 24-1strain,24-2strain,1804001strain, 1806003strain,and DYN strain,were isolated from 3outbreaks in the military forces stationed around Chengdu City.The whole genome sequences of these 5HAdV-55strains (GenBank accession numbers MH256653,MH256554,MH255655,MH256656,and MH256657,respectively)obtained by 12-segment fragmentation PCR sequencing showed only minor variations with mutations of 16bases.Compared with the sequence of the first isolated HAdV-55strain (QS-DLL)in China,these strains showed mutations at 30 bases.Phylogenetic tree analysis showed that the 5newly isolated HAdV-55strains were similar to the QS-DLL strain and the Chongqing strain (CQ-2903),and their evolutionary distances were greater from the previously isolated Tibet strain (LS89/Tibet/2016)and Yunnan strain (KM03/YN/2016).Conclusion The HAdV- 55strains prevalent in the 2018outbreaks in the military forces around Chengdu are QS-DLL-like strains or mutant strains of CQ-2903.
作者
王文博
古良琪
刘媛
杨洋
赵秀华
周奕帆
张雪莲
陈锚锚
邱薇
范泉水
胡小兵
WANG Wenbo;GU Liangqi;LIU Yuan;YANG Yang;ZHAO Xiuhua;ZHOU Yifan;ZHANG Xuelian;CHEN Maomao;QIU Wei;FAN Quanshui;HU Xiaobing(Center for Disease Control and Prevention of Western Theater Command,Chengdu,Sichuan Province,610021,China;Department of Clinical Laboratory,.the General Hospital of Western Theater Command,Chengdu,Sichuan Province,610083,China)
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2018年第24期2211-2216,共6页
Journal of Third Military Medical University
基金
中国博士后科学基金(2018T111157
2017M623427
2017M613427
2017T100820)~~
作者简介
通信作者:范泉水,E-mail:13888254888@126.com;通信作者:胡小兵,E-mail:18981715872@189.cn
