摘要
为研究鸡白痢沙门菌双组份系统CpxR/A的作用,本研究利用λ-Red重组技术构建了鸡白痢沙门菌cpxR基因缺失突变株ATCC19945ΔcpxR,通过应激试验和细胞感染试验,揭示cpxR基因在鸡白痢沙门菌致病机制中的重要作用。实验结果表明,cpxR基因缺失并未影响细菌的生长速度、生化特性和在鼠源巨噬细胞Raw264.7中的存活能力,但使得细菌对热、酸、碱和氧化应激的敏感性增加,同时在蛋清中的生存能力下降。本研究为进一步阐释CpxR/A系统的重要功能奠定基础。
To investigate the roles of two-component regulatory systems CpxR/A of Salmonella enterica serovar pullorum,the cpxR gene was knocked out from S.pullorum ATCC19945strain by λ-Red recombination,and examined by stress test and cell infection test for analysis its contribution to pathogenic mechanism of S.pullorum.The data demonstrated that the CpxR-deficient mutant showed no difference in growth characteristics,biochemical characteristics and survival ability in Raw 264.7,compared with wild type strain.However,the mutant strain showed increased sensibility to environment stress including heat,acid,alkali, oxidative and survival defection in albumen.This study provided the basis for exploring underlying mechanism of CpxR/A system.
作者
吴同垒
于秀剑
黄翠琴
张志强
李佩国
朱国强
WU Tong-lei;YU Xiu-jian;HUANG Cui-qin;ZHANG Zhi-qian;LI Pei-guo;ZHU Guo-qiang(Yangzhou University,Yangzhou 225009,China;Hebei Normal University of Science &Technology,Qinhuangdao066004,China;Fujian key laboratory of Prevention and control of livestock contagious disease &biotechnology,Longyan364000,China)
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2018年第11期981-985,共5页
Chinese Journal of Preventive Veterinary Medicine
基金
河北省现代农业产业技术体系蛋鸡产业创新团队岗位项目(HBCT2013090203)
福建省家畜传染病防治与生物技术重点试验室开放基金课题(ZDSYS2017001)
河北科技师范学院博士启动基金(2015YB001)
作者简介
吴同垒(1987-),男,山东东平人,讲师,主要从事动物病原微生物学研究;通信作者:李佩国E-mail:lpeiguo@163.com;通信作者:朱国强,E-mail:yzgqzhu@hotmail.com.
