摘要
地下水位波动带氧化还原条件交替变化,是物质循环最活跃的场所,也是物质从地表向地下迁移的必经之路。研究地下水位波动带氮循环功能菌群的特性对弄清氮素循环、控制地下水氮污染具有重要的意义。该文利用高通量16S rDNA的测序技术对江汉油田地下水位波动带土壤中氮循环功能菌群进行分析和研究。结果表明,氮肥施用和多环芳烃污染共同决定波动带微生物种类多寡和各种氮循环功能菌群的消涨。多环芳烃抑制了整体微生物群落的活性,尤其是反硝化菌群;农田中氮肥施用后氨氮含量较高,硝化菌群占主导地位;由于硝酸盐容易迁移至下层,造成波动带硝态氮含量较高,反硝化菌群随之增加。
Groundwater table fluctuation zone is not only a most active place for material cycling due to the redox alterna- tion,but also an essential pathway for materials transporting from surface to subsurface.It is of significance to study the char- acteristics of nitrogen cycling bacterial flora in this zone so as to clarify nitrogen cycle and control nitrogen pollution in groundwater.The present paper used 16S rDNA sequencing technology to study the nitrogen cycling microbial flora in the soil of groundwater table fluctuation zone in the Jianghan Oilfield'.Results showed that nitrogen fertilizer and polycyclic aromatic hydrocarbon (PAHs)controlled the microbial abundance and fluctuation of nitrogen cycling functional microbes in groundwa- ter table fluctuation zone.PAHs inhibited the activity of microbial community,especially denitrifying bacterial flora.In agri- cultural field,nitrogen fertilizer provided high concentration of NH4^+-N,nitrifying bacterial flora became abundant.Due to the transport of nitrate more easily,nitrate concentration in the groundwater fluctuation zone of agricultural field was higher,and denitrifying bacterial flora increased accordingly.
作者
赵磊
刘慧
ZHAO Lei;LIU Hui(State Key Laboratory of Biogeology and Environmental Geology,School of Environmental Studies,China University of Geosciences,Wuhan 430074,China)
出处
《环境科学与技术》
CAS
CSCD
北大核心
2018年第11期49-53,共5页
Environmental Science & Technology
关键词
波动带
氮转化
多环芳烃
功能微生物
16SrDNA
groundwater table fluctuation zone
nitrogen transformation
polycyclic aromatic hydrocarbon (PAHs)
function- al microbes
16S rDNA
作者简介
赵磊(1993-),男,硕士研究生,研究方向为环境化学生物学,(电子信箱)2423616595@qq.com;刘慧,通讯作者,(电子邮箱)hliu2009@cug.edu.
