摘要
目的评价云南草果居群的遗传多样性及亲缘关系。方法运用7对微卫星引物对24个草果居群进行分析;首先应用GenALEx计算遗传多样性参数,并进行PCoA和AMOVA分析;采用NTsys软件绘制居群聚类图;最后利用Structure软件计算出最佳的K值。结果 24个草果居群的Shannon多样性指数(H)的平均值为0.49,期望杂合度(He)的平均值为0.32;遗传分化系数(Fst)为0.090,基因流(Nm)为2.930。24个草果居群的遗传分化有81%存在于居群内,仅有19%存在于居群间;黄花草果23个居群的遗传一致度(I)为0.631 8～0.982 4,遗传距离(D)的范围为0.017 7～0.459 2,而白花草果居群(MG5)与其他23个黄花草果居群一致度均较小,为0.3697～0.6090;而居群聚类分析,在遗传距离0.49处,明显地把白花草果与黄花草果分开;Structure聚类得出K=4时,209份黄花草果资源可被分为4个类群。结论云南黄花草果居群的遗传多样性水平平均偏高;遗传变异主要存在于居群内,而非居群间。根据基因型,黄花草果和白花草果被明显地划分成2类,遗传距离很远;而黄花草果大致被分为4个类群。
Objective To evaluate the genetic diversity and phylogenetic relationships of Amomun tsao-ko populations in Yunnan. Methods Seven pairs of microsatellite(SSR) primers were used to analyze 24 A. tsao-ko populations; First, GenALEx was used to calculate genetic diversity parameters, PCoA and AMOVA analysis was carried out; NTsys software was then used to draw population clusters map; And finally, the Structure software was used to calculate the best K value. Results The average of Shannon’s diversity index(H) of the 24 A. tsao-ko populations was 0.49, the average of heterozygosity(He) was 0.32, the genetic differentiation coefficient(Fst) was 0.090, and the gene flow(Nm) was 2.930. Eighty-one percent of the genetic differentiation among the 24 populations of A. tsao-ko existed within the population, and only 19% existed among the populations. The genetic identity(I) of the 23 A. tsao-ko populations of yellow flowers was 0.631 8—0.982 4. The genetic distance(D) was in the range of 0.017 7— 0.459 2, while the consistency degree of the A. tsao-ko population of white flower(MG5) and 23 other yellow flowers was 0.369 7—0.609 0. However, cluster analysis showed that A. tsao-ko population of the white flowers and yellow flowers were clearly separated at the genetic distance of 0.49. Structure clustering showed 209 A. tsao-ko resources can be divided into four groups when K value was 4. Conclusion The genetic diversity of A. tsao-ko populations of yellow flowers of Yunnan is higher on average, and the genetic variation is mainly found in population rather than among populations. According to the genotypes, A. tsao-ko of yellow flower and white flower are clearly divided into two categories, and the genetic distance is very far; and the yellow flower of A. tsao-ko is roughly divided into four groups.
作者
胡一凡
张雪梅
徐绍忠
杨生超
杨志清
HU Yi-fan;ZHANG Xue-mei;XU Shao-zhong;YANG Sheng-chao;YANG Zhi-qing(College of Agronomy and Biotechnology,Yurman Agricultural University,Kunming 650201,China;Key Laboratory of Medicinal Plant Biology of Yunnan Province,Yunnan Agricultural University,Kunming 650201,China;National&Local Joint Engineering Research Center on Germplasm Innovation &Utilization of Chinese Medicinal Materials in Southwestern China,Yunnan Agricultural University,Kunming 650201,China;Yurman Engineering Technology Research Center for Aromatic Biology,Ktmming 650201,China)
出处
《中草药》
CAS
CSCD
北大核心
2018年第22期5388-5395,共8页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金项目"草果居群的分子鉴定及种质资源评价"(81560615)
云南省社会发展科技计划项目"云南草果种植加工关键技术研究与示范"(2011CG015)
云南省科技惠民计划项目"富宁石漠化区域中药材种植关键技术研究及应用示范"(2015RA083)
云南省科技计划项目"云南省芳香生物工程技术研究中心建设"(2018DH010)
关键词
草果
简单重复序列分子标记
遗传多样性
亲缘关系
聚类分析
Amomun tsao-ko Crevost et Lemaire
SSR
genetic diversity
genetic relationship
clustering analysis
作者简介
胡一凡(1994一),女,河北承德人,硕士研究生,研究方向为药用植物资源分子鉴定。Tel:13187708818E—mail:huyisarah@163.com;通信作者,杨志清,硕士生导师,副教授,主要从事药用植物栽培与资源评价研究。E-mail:yzql468@126.com.
