摘要
对一株分离自萝卜 ( Raphanus sativus)的黄瓜花叶病毒 ( CMV)卫星 RNA( Rs)进行了 c DNA克隆与序列确定 ,并与 12个已知的卫星 RNA序列进行了比较 .结果表明 :卫星 RNA Rs的大小为 368nt;该卫星 RNA所比较的 13个卫星 RNA的大小分布为 334～ 4 0 5nt,可分为 3个组和 4个亚组 ;CMV卫星 RNA的序列同源性和分组与卫星 RNA的大小并无直接联系 .卫星 RNA序列之间的连续缺失和插入均集中在 90～ 2 55nt间几个邻近的区域 .在卫星 RNA二级结构中的非配对区序列、卫星 RNA的 5'端近 80个碱基和 3'端近 4 5个碱基相当保守 .由此推测 CMV卫星 RNA的理论长度可达 4 4 6nt.序列结构的比较显示 ,CMV卫星 RNA具有作为外源基因载体的潜力 .
WT5”BZ]The sequence of a sat RNA of cucumber mosaic virus (CMV Rs) originally isolated from Raphanus sativus in Hangzhou, Zhejiang Province in April 1998, was determined. This satllite was found consisting of 368 nucleotides (nt). Compared its nucleotide sequence with those of 12 CMV sat RNAs in document, ranging from 334 nt to 405 nt, resulted three groups and four subgroups. The sequence similarity and grouping were not related to the sizes of the sat RNAs. The sites of nucleotide insertion or deletion on the 13 sat RNAs were located at several neighboring sections between 90 nt and 255 nt. The 80 nt at the 5' end, the 45 nt at the 3'end, and unpaired sections in satellite RNA structure were highly conservative. It is thus speculated that a CMV sat RNA may include 446 nt and could be highly potential for vectoring a foreign fragments for particular purpose. [WT5”HZ]
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2001年第3期249-254,共6页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
国家自然科学基金资助项目! (39880 0 2 8)
浙江省重大科技资助项目!(99110 6 2 0 6 )
