摘要
探讨了卡那霉素 (Kan)、羧苄青霉素 (Cb)、头孢霉素 (Cef)和氨苄青霉素 (Amp) 4种抗生素对台湾青枣幼嫩茎段和疏松的愈伤组织生长的影响 ,初步确立了对台湾青枣 (ZiziphusmauritianaLam .)进行农杆菌介导的遗传转化研究中的抑菌和转化体的筛选策略 .Kan 75mg·L-1时 ,5 0d后茎段基本停止生长并开始变成白色 (白化 ) ;Kan2 5mg·L-1时 ,胚性愈伤组织生长受到明显的抑制 ,Kan 5 0mg·L-1时 ,其生长基本停止且开始褐变 ,Kan 75mg·L-1以上时胚性愈伤组织完全变褐 .适当浓度的Cb或Cef对芽的伸长生长有一定的促进作用 ,Cb +Cef却对芽的伸长出现轻微的抑制效应 .
Effects of antibiotics, including kanamycin (Kan), carbenicillin (Cb), cefotaxime (Cef), ampicillin (Amp), on growth of stem segments and callus of ber( Ziziphus mauritiana Lam. var. Gaolang No. 1 ) were studied. Strategy of bacterium inhibition and GMOs(genetically modified organism) selection of ber has been determined initially in agrobacterium mediated genetic transformation. Stem segments would stop growing and become white after 50 d in Kan 75 mg·L -1 treatment;Embryogenic Callus growth would be inhibited significantly by Kan treatment at 25 mg·L -1 ,and would be suspended and start to turn brown at 50 mg·L -1 ,and would be brown completely at 75 mg·L -1 or above. Moderate concentration of Cb or Cef could promote growth of bud at certain degree,Cb+Cef would have a slight inhibition to bud elongation. Amp played a role of inhibition for stem segment growth.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2001年第2期21-23,共3页
Journal of South China Agricultural University
基金
华南农业大学陈励文基金资助项目! (5 30 0 k990 47)
