摘要
目的 应用高效液相色谱法测定血清中氯硝西泮血药浓度及对癫痫患儿的血药浓度进行监测。方法 采用Nova PakC18色谱柱 ,乙腈 0 .1mol·L-1醋酸钠 =35∶6 5作流动相 ,紫外检测波长 2 5 4nm ,苯妥英钠为内标 ,用叔丁基甲醚提取 ,常温下空气吹干 ,重溶进样分析。结果 氯硝西泮在 5 .0～ 10 0 .0 μg·L-1浓度范围内线性关系良好 ,相关系数为 0 .9990 ,在 3种不同浓度下 ,平均相对回收率为 99.80 % ,99.34 % ,99.6 2 % ,绝对回收率为 91.96 % ,92 .16 % ,90 .0 6 % ,日内RSD为 1.41% ,3.76 % ,3.30 % ,日间RSD为 1.5 8% ,1.71% ,4.73% ,最低检出限为 2 .0 μg·L-1血清。该方法监测儿科临床口服氯硝西泮达稳态的癫痫患儿 11例 ,血药浓度为 13.0 4～ 39.17μg·L-1。
OBJECTIVE: To establish a HPLC method for determining the serum clonazepam concentrations in epileptic children. METHODS: With Nova-Pak C18 column, the mobile phase was consisted of 35% acetonitrile and 65% 0.01 mol · L-1 sodium acetate. Phenytoin was used as the internal standard. It was extracted by tertbutylanisole and drying at room temperature. The residue was reconstitued by the mobile phase and then analyzed at UV 254 nm. RESULTS: The good linearity was obtained over the range of 5.0 ∼ 100.0 μg · L-1 with correlation coefficient r = 0.9990. At three concentrations, the average recoveries were 99.80%, 99.34% and 99.62%, respectively. RSD of within-day were 1.14%, 3.76% and 3.30%, respectively. RSD of between-day were 1.58%, 1.71% and 4.73%, respctively. The minimal detectable concentration was 2.0 μg · L-1. The steady state serum concentrations were 13.04 ∼ 39.14 μg · L-1 in 11 epileptic children after oral administration of clonazepam. CONCLUSION: This method can provide the basis for clinical effective and safe treatment of clonazepam.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2001年第7期470-472,共3页
Chinese Pharmaceutical Journal
关键词
氯硝西泮
高效液相色谱法
血药浓度
监测
抗惊厥药
Acetonitrile
Concentration (process)
Liquid chromatography
Patient treatment
