摘要
目的 探讨端粒酶、p5 3突变蛋白作为肺癌肿瘤标记物的可能性以及二者与细胞增殖DNA合成期比例(SPF值 )的关系。方法 采用改良银染 -端粒重复序列扩增法 (TRAP)检测 4 0例原发性支气管肺癌端粒酶活性 ,免疫组织化学方法检测其p5 3突变蛋白 ,流式细胞术检测其SPF值。结果 87.5 % (35 /40 )肺癌组织端粒酶活性阳性 ,92 .5 % (37/40 )p5 3突变蛋白阳性 ;SPF值在端粒酶活性阴性组、阳性组之间 ,p5 3突变蛋白阴性组、阳性组之间均有显著性差异。结论 端粒酶、p5 3突变蛋白可能是灵敏、特异的肺癌肿瘤标记物。端粒酶的异常活化、表达主要发生在细胞分裂周期的S期 ,p5
Objective To identify the oncological significance of telomerase activity and p53 mutation protein in human lung cancer and investigate the associations of them with S-phase fraction (SPF) in lung cancer. Methods The techniques of silver staining-telomeric repeat amplification protocol (TRAP), immunohistochemistry and flow cytometry (FCM) were employed to examine the telomerase activity, p53 mutation protein and SPF in 40 lung cancer specimens. Results Telomerase activity and p53 mutation protein were detected in 87.5% (35/40) and 92.5% (37/40) of lung cancer specimens respectively. Significant difference of SPF was found between telomerase activity positive and negative groups, and between p53 mutation protein positive and negative groups. Conclusion Telomerase and p53 mutation protein may be two sensitive and specific tumor markers of lung cancer. Telomerase activity might be abnormally activated and mainly expressed in the S phase of cell cycle, and p53 mutation protein might take part in the regulation of telomerase activity.
出处
《徐州医学院学报》
CAS
2002年第1期30-33,共4页
Acta Academiae Medicinae Xuzhou
基金
江苏省科委基金资助项目(BJ98110 )
江苏省教委基金资助项目(JW970 0 4 5 )
江苏省卫生厅基金资助项目(H980 8)
徐州市科委(X970 2 3)基金资助项目
