摘要
为了进一步明确 ＨＢＶ基因组 Ｓ区编码产物对乙型病毒性肝炎诊断的临床价值，用双抗体夹心 ＥＬＩＳＡ法检测 ９０份乙型病毒性肝炎患者血清标本ＨＢｓＡｇ·ＰＨＳＡ－Ｒｅ、Ｐｒｅ－Ｓ１和 Ｐｒｅ－Ｓ２，并与 ＨＢＶＭ及 ＨＢＶＤＮＡ ＰＣＲ进行对比分析。结果以抗 ＨＢｃ、ＨＢｓＡｇ·ＰＨＳＡ－Ｒｅ、ＨＢＶＤＮＡ（ＰＣＲ）及 Ｐｒｅ－Ｓ２的阳性检出率为最高，分别为８７．８％、７３．３％、７２．２％和６４．４％，以ＨＢＶＤＮＡ作为ＨＢＶ感染的金标准，无显著性差异（Ｐ＞０．０５）；其中ＨＢｓＡｇ·ＰＨＳＡ－Ｒｅ与 ＨＢＶＤＮＡ和ＨＢｅＡｇ的符合率最高，分别为 ７６．７ ％和 ７４．７ ％；ＭＢｓＡｇ·ＰＨＳＡ－Ｒｅ与 ＨＢＶＤＮＡ具有高度相关性。ＨＢｓＡｇ·ＰＨＳＡ－Ｒｅ做为早期诊断ＨＢＶ感染及判断是否具有传染性方面，是一项非常重要的血清学指标，Ｐｒｅ－Ｓ２也可作为ＨＢＶ复制活跃的标志之一，用于急慢性肝炎的预后判断．
To understand the clinical value of encoding product by pre- S region genes of hepatitis B virus (HBV) on diagnosing for hepatitis B, HbsAg PHSA- Re, Pre- S1 and Pre-S2 of 90 serums of patient with HBV infection were determined by enzyme linked immunoassays (ELIAS), and compared with HBV serologic marker (HBVM) and HBVDNA(PCR). The results showed that the positive rates of anti - HBc, HBsAg PHSA - Re, HBVDNA and Pre -S2 are 87.8%, 73.3%, 72.2% and 64.4%, respectively HBV DNA (PCR) is the best accordant for infection of HBV Our finging suggests that HBeAg PHSA - Re is an impartant serologic marker for diagnosing early and monitoring HEV in fection, and that Pre -S2 is as well important for observing prognosis of acute and chronic HBV infection.
出处
《临床肝胆病杂志》
CAS
北大核心
2002年第1期33-34,共2页
Journal of Clinical Hepatology
