摘要
目的 探讨高血压病患者早期肾脏损害的诊断方法。方法 采用速率散射比浊法检测尿微量白蛋白 (mALB)、β2 微球蛋白 (β2 MG)、全定量酶免疫法测定尿视黄醇结合蛋白 (RBP)、速率法检测N 乙酰 β D 氨基葡萄糖苷酶 (NAG)、Jaffe速率法测定尿肌酐。 结果 高血压病患者尿RBP、mALB、β2 MG、NAG均较对照组显著增高 (P <0 .0 1) ,并随病期的延长有逐渐增高的趋势。单项及二项检测RBP、mALB、β2 MG、NAG阳性率较低 ,联合其中三项检测阳性率较高 ,联合四项检测阳性率可达85 1% ,RBP与 β2 MG、NAG呈显著正相关 (P <0 .0 5 )。mALB与RBP、β2 MG、NAG无相关性 (P >0 0 5 )。结论 检测尿RBP、mALB、β2 MG、NAG是诊断高血压病早期肾损害的敏感指标 ,联合三至四项指标有较高的检出率 ,对于高血压肾病的早期诊断 。
Objective To study diagnostic methods for early renal injury in hypertension patients. Methods Urinary microalbumin (mALB) and β 2 microglodulin(β 2 MG) levels were measured with rate nepherometry. Total quantitative enzyme immunoassay was employed to measure urinary retinol binding protein (RBP) levels, rate for urinary N acetyl beta D glucosaminidase (NAG), and Jaffes rate for urinary creatinine (Cr). Results The levels of urinary RBP, mALB, β 2 MG, NAG in hypertension patients were significantly higher than those in controls ( P <0.01). The levels were increased gradully with the prolongation of patients condition. Measuring four markers together would raise the positive rate to 85.1% in hypertension patients. A significant positive correction was noted between urinary RBP and β 2 MG, NAG, but no correction between mALB and RBP, β 2 MG, and NAG. Conclusions The method is sensitive to measure urinary RBP, mALB, β 2 MG and NAG in the diagnosis of early renal injury in hypertension patients. Measuring three or four markers together can get a higher positive rate. It is of value in detecting degree of injury to kidney in patients with hypertensive nephropathy.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2001年第5期282-284,共3页
Chinese Journal of Laboratory Medicine
