摘要
用盐酸水解、乙醚提取、醋酐、吡啶乙酰化衍生后 ,加入内标物质环戊醚雌酮 ,应用GC MSD对人尿中的雌三醇作定性定量测定 ,用该法对事故放射病人药后尿中排出量进行检测 ,实验结果显示E3(OAc) 3在尿中以原形排出 ,排泄曲线表明主要排出在前三天。
Estriol (E 3) was qualitatively and quantitatively determined by GC/MSD with an internal standard, 3-cyclopentanoxy-estrone (CE 1). Urine sample was hydrolyzed with hydrochloric aid and then extracted with ether. Finally it was derived with acetic anhydride and pyridine. The method was used in determination of the urine of 2 patients who were suffered from radiation accidents and then treated with E 3. The results showed that E 3(OAc) 3 was excreted as its prototype and the excretion was mainly excreted during first three days.
出处
《质谱学报》
EI
CAS
CSCD
2001年第3期47-50,共4页
Journal of Chinese Mass Spectrometry Society
