摘要
目的 探讨癫痫鼠脑组织中 P5 3及 Bcl-2的表达及意义。方法 通过免疫组化方法对青霉素致痫鼠癫痫灶脑组织中的 P5 3 、Bcl-2进行检测。结果 随着痫性发作次数的增多 ,P5 3 有显著的上升趋势 ;致痫组有 Bcl-2的表达 ,发作 8次组 Bcl-2表达最高 ,以后随着痫性发作次数的增多 ,有显著的下降趋势 ;P5 3 基因与 Bcl-2蛋白表达呈负相关性。结论 P5 3 基因表达水平与神经元损害程度有一定的关系。P5 3 基因可诱导 Bcl-2表达的减少 ,神经细胞的自我保护是有限的。
Objective To investigate the change of P 53 and Bcl 2 in cerebral tissues induced by seizures.Methods The model of seizure in Wistar rats was performed by injecting penicillini intraperitoneally.The levels of P 53 and Bcl 2 were detected by immunohistochemistry.Results (1)The level of P 53 in the epileptic group was significantly higher than that in the control group (P<0.01).The level of P 53 in the hippocampus is significantly higher than that in the cerebral cortex in the epileptic group (P<0.05).(2)There was no expression of Bcl 2 in the control group . There was expression of Bcl 2 in the epileptic group . The level of Bcl 2 in the hippocampus was significantly higher than that in the cerebral cortex (P<0.01). The level of Bcl 2 in the epileptic group with eight onsets was highest(P<0.01).(3)There was an inverse relationship between the expression of P 53 and that of Bcl 2.Conclusion Increased P 53 expression corrlates with the degree of neuronal injury . The change of Bcl 2 protein in the brain cells may protect the cells from injury and apoptosis. But the protection role of Bcl 2 is limited .Increase of P 53 expression may induce the decrease of Bcl 2 expression.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2001年第4期220-222,共3页
Journal of Apoplexy and Nervous Diseases
