摘要
本文在增效作用测定基础上 ,研究比较了多功能氧化酶系 (MFO)的三个组分 (细胞色素 P4 50、细胞色素 b5、细胞色素 c还原酶 )在相对敏感棉铃虫 (HDS)与抗氰戊菊酯棉铃虫 (KQR)中的含量、活性及其组织分布规律。增效作用测定结果表明 ,PBO及 SV1对氰戊菊酯具有显著的增效作用 ,增效倍数分别达 2 53.99和71.4 4倍 ;初步表明 MFO在 KQR种群棉铃虫的抗药性中起着重要作用 ;随后的生化研究表明 :KQR种群棉铃虫中肠、脂肪体及体壁的三个组分含量均显著或极显著高于 HDS种群棉铃虫相应部位的含量 ,其中细胞色素 P4 50在上述三个部位中的含量分别是 HDS种群棉铃虫的 1.76、3.12、4 .15倍 ,细胞色素 b5含量分别是HDS种群棉铃虫的 1.57、6 .2 8、2 .39倍 ,细胞色素 c还原酶活性分别是 HDS种群棉铃虫的 1.4 0、1.88、1.6 4倍 。
Synergism of PBO and SV 1 and 3 major components(cytochrome P450, cytochrome b 5, cytochrome c reductase) of mixed-function oxidases (MFO) and its tissue distribution were compared between a fenvalerate-resistant strain (KQR) and a susceptible strain (HDS) of -Helicoverpa armigera-. Both PBO and SV 1 significantly increased the toxicity of fenvalerate to KQR strain, the synergistic ratio(SR) was 253.99- and 71.44- fold respectively, suggesting the important role of MFO in the resistance of KQR strain of -H. armigera.- The total cytochrome P450, cytochrome b 5, NADPH-cytochrome c reductase of KQR strain were 1.76-, 1.57-, 1.40-fold higher than HDS strain in midgut microsomes, 3.12-, 6.28-,1.88-fold higher in fat body microsomes and 4.15-, 2.39-, 1.64-fold higher in integument microsomes, respectively. The study also showed the tissue distribution patterns of the three components.
出处
《农药学学报》
CAS
CSCD
1999年第2期54-60,共7页
Chinese Journal of Pesticide Science
