摘要
从家蚕品种大造和C108及其F1,F2的后部丝腺提取的基因组DNA通过RAPD扩增,在477个随机引物中,85.9%的引物在家蚕基因组有稳定的扩增产物,总扩增片段5155条,每个引物扩增片段1～23条,平均为12.6条,多态性片段数共1496条,占两品种中总片段数的29.0%,经多次重复及反复验证发现,只要严格控制扩增反应条件及保证基因组DNA和试剂等不污染,则能检测到稳定的DNA多态性片段,从而保证其稳定性与重复性。
The DNAs, taken from the hind silk gland of Silkworm C 108 , Dazao and from their first and second generations, were used for amplification by means of RAPD and it showed that 85.9% of the 477 random primers had stable products. The total number of amplified bands was about 5 155, with the bands of each amplifier ranging from 1 to 23, which averaged in 12.6. The polymorphic DNA fragments observed were 1 496, which was 29.0% of the total amplified products. After extensive repeat and experiments on large scale, it strongly suggested that only in a strict amplified condition and without any pollution in every step did the DNA polymorphism show repeatability and stability.
出处
《西南农业大学学报（自然科学版）》
CSCD
1999年第5期408-411,共4页
Journal of Southwest Agricultural University
基金
国家自然科学基金!(39730 370 )
教育部博士点专项基金!(96 0 6 0 2 )
