摘要
为优选葛根素的提取工艺 ,本实验采用 4因素 3水平正交设计法 ,以葛根素为指标 ,筛选影响葛根醇提工艺的因素。结果表明实验设计 4因素均具有显著性影响。结合生产实际需要 ,葛根最佳提取条件是 :加入 18倍量 80 %乙醇回流提取 3次 (8,6 ,4 ) ,提取 5h(2 ,2 ,1)。
In order to optimize the technology of extracting the isoflavone constituents from pueraria root, the experiment used the orthogonal experimental design (4 factors and 3 levels) with the content of puerarin as an experimental criterion in screen the factors affecting the technology of extracting the isoflavone constituents from pueraria root. The results showed that all the 4 factors designed in the orthogonal experiment signifantly affected the extracting technology. In consideration of the industrial practice, the best condition of extracting the isoflavone constituents from pueraria root was decided: reflux extraction with 80% alcohol for 3 times, i.e. extracting with 8 fold alcohol for 2 hours for the first time, with 6 fold alcohol for 2 hours for the second time, and with 4 fold alcohol for 1 hour for the third time.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2001年第4期26-27,55,共3页
Journal of Beijing University of Traditional Chinese Medicine
关键词
正交试验
葛根素
提取工艺
Orthogonal Experiment
Pueraria Root
Puerarin