摘要
目的 :为了比较三种巧克力平板痰培养流感嗜血杆菌的检出率和生长情况。方法 :将送检的痰标本及流感嗜血杆菌标准菌株ATCC 1 0 2 1 1分别接种于羊血、马血和兔血巧克力平板上 ,置烛缸中 ,36℃培养 2 4h ,比较流感嗜血杆菌检出率及其生长情况。结果 :①三种巧克力平板痰培养流感嗜血杆菌的检出率分别为 8%、 34 %及 36 %。②标准菌株ATCC 1 0 2 1 1在马血和兔血巧克力平板上生长良好 ,菌落直径 0 5～ 1mm ,而在羊血巧克力平板上生长差 ,菌落大小如“沙粒” ,肉眼难见。结论 :痰培养流感嗜血杆菌应使用马血或兔血巧克力平板 ,而不用羊血巧克力平板。
Objective:To compare the isolate rates of Haemophilus influenzae in sputum by chocolate platee made of sheep blood,horse blood or rabbit blood,and the growth of Haemophilus influenzae on the thee kinds of chocolate plates Methods: Sputums and Haemophilus influenzae ATCC 10211 were streaked on the three kinds of chcocolate plates Incubate at 36℃ in a candle jar for 24 hours,The isolate rates of Haemophilus influenzae by three kinds of chocolate plates and the growth of Haemophilus influenzae ATCC 10211 on them are compared Results:(1)The isolate rates of Haemophilus influenzae in sputum by chocolate plates made of sheep blood,horse blood or rabbit blood were 8%、34%、36% respectively (2)Haemophilus influenzae ATCC 10211 grows very well on chocolate plates made of horse blood or rabbit blood,Its diameter of colony is 0 5 1 0 mm But it does not grow well on the chocola plates made of sheep blood,Its colony is as small as a sand and hard to be seen with naked eye Conclution:Chocolate plates made of horse blood or rabbit blood can be used to isolate Haemophilus influenzae in sputum But the chocolate plate made of sheep blood can not
出处
《广州医药》
2001年第3期64-65,共2页
Guangzhou Medical Journal
关键词
动物血
巧克力平板
流感嗜血杆菌
检出率
Animal blood
Chocolate plate
Haemophilus influezae
Isolate rate
