摘要
目的通过考察灵芝多糖（ＧＬＰ）对免疫细胞信号转导过程的影响，探讨ＧＬＰ免疫调节作用机制。方法采用激光扫描共聚焦显微镜（ＬＳＣＭ）技术，动态监测ＧＬＰ均一体组分ＧＬＢ７对小鼠Ｔ细胞胞浆游离Ｃａ２＋浓度（［Ｃａ２＋）和胞内 ｐＨ（［ｐＨ］ｉ）的影响。结果 ＧＬＢ７（２０ｍｇ·Ｌ－１）引起小鼠 Ｔ细胞中［Ｃａ２＋］；和［ｐＨ］；明显升高，１ｍｉｎ即分别升高至３３４．７０％±１６，４％（ｎ＝３）、１７１．６％ ± １０．４％（ｎ＝３），之后一直分别维持在该平台期，至 １０ ｍｉｎ仍维持高峰水平。加入ＥＣＴＡ和 ｖｅｒａｐａｍｉｌ处理后， １０ ｍｉｎ ＧＬＢ７ 引起 Ｔ细胞［Ｃａ２＋］；和［ｐＨ］；分别升高为 ２０２．４％± １３．６％（ｎ＝３）。１４０．２％±７．８％（ｎ＝３），与正常对照组比较差异有显著性（Ｐ＜０．０1）。以 ＥＧＴＡ和 ｖｅｒａｐａｍｉｌ处理后，再分别以 ｈｅｐ－ｅｒｉｎ和 ｐrocaine处理细胞 １０ ｍｉｎ，之后以 ＧＬＢ７刺激Ｔ细胞，１０ｍｉｎ［Ｃａ２＋］ｉ值分别为１５１．５％±９．４％（ｎ＝３）、１４３．２％± ８．１ ％（ ｎ＝ ３），与 ＥＧＴＡ和 ｖｅｒａｐａｍｉｌ处理组相比差异有显著性（ Ｐ＜ ０．０１）。
AIM To extend the approach of the action of ganoderma polysacchride on intracellular signal events in T cells. METHODS Laser scanning confocal microscope imaging of the calcium and pH fluorescent indicator dye Fluer3/AM and SNARF-1/ AM were used to determine the kinetic changes of [Ca2+ ], and [pH], in murine T cells induced by a ganoderma polysacchride, designated GLB7.RE- SULTS it was found that GLB7(20 mg.L-1 ) could increase [Ca2+ ], and [PH], at 1 min were 334.7% ± 16.4%(n = 3).171.6% ± 10.4% (n = 3) respectively. The increase in [Ca+ ]i induced by GLB7 was due to the influx of extracellular Ca2+ and intracellular Ca2+ release through ho lP3-sensitive and IP3- insensitive Ca2+ stores, and increase in [PH]i induced by GLB7 was relative to Na+ /H+ exchange systems and [Ca2+ ]i. GLB7 did not influence [Ca2+ ], and [pH], in murine T cells induced by Con A(3 mg· L-1 ). CONCLUSION Stimulation of the increase in [Ca2+ ], and [pH], may be an important channel for ganoderma polysacchrides to achieve their pharmacological actions.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2001年第2期167-170,共4页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助课题
No 39400165
关键词
灵芝多糖
T细胞
游离胞浆Ca^2+浓度
PH值
激光扫描共聚焦显微镜
ganoderma lucidum
polysaccha- ride
T cell
intracellular free calcium
intracellular pH
laser scanning confocal microscope
