摘要
目的 研究维生素E琥珀酸酯 (VES)对人胃癌细胞(SGC 790 1)生长以及DNA合成的影响。方法 以体外培养的人胃癌细胞 (SGC 790 1)作为研究对象 ,用活细胞计数方法绘制生长曲线。Giemsa染色方法计数细胞形成集落数。用流式细胞仪检测VES对SGC 790 1细胞周期的影响。并用3H TdR参入方法研究VES对SGC 790 1细胞DNA合成的影响。结果 VES可抑制SGC 790 1细胞的生长和集落形成 :5mg·L-1、10mg·L-1和 2 0mg·L-1VES处理SGC 790 1细胞 7d后 ,细胞生长抑制率分别为 41 2 %、98 3%和 10 0 % ;同样剂量的VES处理SGC 790 1细胞 2 4和48h后 ,集落形成抑制率分别为 6 7%、5 0 4%、87 2 %和2 4 7%、73 4%、10 0 %。细胞周期分析显示VES作用细胞48h后 ,G2 M期细胞比例降低 ,并呈一定的剂量效应关系 ;同时S期细胞比例升高。在培养 2 4和 48h后VES对SGC 790 1细胞3H TdR参入均有明显抑制作用 ,且呈剂量效应关系。
AIM To study the effects of vitamin E succinate (VES) on the cell growth and the DNA synthesis of human gastric carcinoma cell (SGC 7901). METHODS The growth curve was determined with counting viable cell numbers. The colony formations were counted with Giemsa dye staining. The cell cycle was analyzed using flow cytometry (FCM) and the DNA synthesis was observed with the 3H TdR incorporation method. RESULTS VES could inhibit the growth and colony formation of SGC 7901 cells. Growth curve display:after SGC 7901 cells were treated with 5 mg·L -1 、10 mg·L -1 and 20 mg·L -1 VES for seven days, the inhibition rate are 41 2%、98 3% and 100%, respectively. The colony formation of SGC 7901 cell at 24 h was inhibited 6 7%、50 4%、87 2%, and at 48 h was 24 7%、73 4%、100%, respectively. FCM analysis revealed that VES could decrease the percentage of cells in G 2 M phase after treated 48 h in a dose dependent manner, while increase the percentage of cells in S pheise. The assays of 3H TdR incorporation into DNA showed obvious inhibition dose dependently after exposure to VES for 48 h. CONCLUSION VES could inhibit gastric carcinoma cell growth by arresting DNA synthesis in vitro .
出处
《中国药理学通报》
CAS
CSCD
北大核心
2001年第1期33-35,共3页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目!No 39870 6 6 2
关键词
胃癌
DNA合成
维生素E琥珀酸酯
vitamin E succinate
human gastric carcinoma cell (SGC 7901)
DNA synthesis
flow cytometry
tritium thymidine incorporation
