摘要
测定了腐霉属的8个种209个菌株对甲霜灵在浓度为0.5和50μg/ml时的抗性,从实验中观察到不同菌株对甲霜灵抗性变化的范围非常大,有95株在0.5μg/ml时,菌丝即停止生长,有20株在50μg/ml时,菌丝生长速度与对照几乎一样。经鉴定,这209株菌中,优势种为终极腐霉(Pythium ultimum)和刺腐霉(P.spinosum)。20株高抗菌中,P.ultimum占16株,P.irregulare占2株,P.spinosum和P.graminicola各占1株。盆栽试验表明:假单胞菌株Pseudomonas sp.CR56对高抗菌株P.ultimum ONCURO1引起的黄瓜苗期猝倒病具有显著的防效,防效与甲霜灵和五氯硝基苯相比,达到极显著差异。
Two hundred and nine isolates of 8 species of Pythium were tested for their sensitivity to metalaxy at concentrations of 0.5 and 50μg/ml, respectively. A large variation in sensitivity of different isolates to metalaxyl was observed. Some isolates did not grow at a concentration of 0.5μg/ ml, while some still grew at a concentration of 50μg/ml at a similar rate of the untreated controls. Twenty isolates grew at a concentration of 50μg/ml, ninety-five isolates did not grow at a concentration of 0.5μg/ml. P. ultimum and P. spinosum dominated the 209 identified isolates. Among 20 isolates which were of high resistance to metalaxy, sixteen were P. ultimum, two was P. irregu-lare, the rest ones were P. spinosum and P. grammicola, respectively. Pot experiments showed that Pseudomonas sp. CR56 had the ability to control cucumber seedlings damping-off disease caused by P. ultimum ONCUR01, which could grow at a metalaxyl concentration of 50 μg/ml. The biocontrol efficacy was significant better than that of metalaxyl and PCNB.
出处
《植物保护学报》
CAS
CSCD
北大核心
2001年第1期55-60,共6页
Journal of Plant Protection
基金
澳大利亚国际农业发展中心(ACIAR)PN9680项目资助
