摘要
本文旨在研究大黄素干预后自发性高血压大鼠(spontaneously hypertensive rat,SHR)脑基底动脉平滑肌细胞BKCa通道电生理特性和表达的变化。在使用大黄素干预处理前后,用尾动脉测压仪观察血压变化,用全细胞膜片钳记录技术观察SHR脑基底动脉平滑肌细胞BKCa通道电流的变化,用免疫组织化学染色和Western Blot观察平滑肌细胞BKCa通道表达变化。结果显示,大黄素干预后,SHR血压由(223±16)mmHg显著降低至(127±12)mmHg(P<0.01),与Wistar大鼠相比没有统计学差异。大黄素显著增加SHR平滑肌细胞外向电流(P<0.05),该增强作用可被BKCa通道特异性阻断剂IbTX所阻断。大黄素干预后SHR基底动脉平滑肌BKCa通道β1亚基表达明显上调(P<0.01)。以上结果提示,大黄素可能通过增加BKCa通道β1亚基表达,增强BKCa通道介导的外向电流,从而发挥舒张SHR脑基底动脉的作用。
The purposes of this study were to investigate the effect of emodin on expression of BKCa channel β1 subunit in basilar artery smooth muscle cells （BASMCs） and electrophysiological characteristics of vascular smooth muscle cells in spontaneously hypertensive rats （SHR）. Tail artery pressure measurement instrument was used to measure the change of SHR systolic blood pressure before and after emodin intervention. Single vascular smooth muscle cell was electrically recorded by whole-cell patch-clamp technique. Immunohistochemistry and Western blotting were used to study the distribution and expression of the BKCa channel β1 subunit. The results showed that emodin decreased blood pressure of SHR from （223 ± 16） mmHg to （127 ± 12） mmHg （P 〈 0.01）. There was no difference of blood pressure between emodin-treated SHR and Wistar rats. Emodin significantly increased outward currents of smooth muscle cells in SHR （P 〈 0.05）, and this effect could be reversed by specific inhibitor of BKCa channel, IbTX. Emodin also up-regulated BKCa channel β1 subunit expression in BASMCs. These results suggest that emodin relaxes cerebral basilar artery by enhancing BKCa current via increasing β1 subunit expression in BASMCs.
出处
《生理学报》
CAS
CSCD
北大核心
2014年第3期289-294,共6页
Acta Physiologica Sinica
基金
supported by National Basic Research Development Program of China(No.2012CB526600)
Xinjiang Graduate Student Research Innovation Project
China(No.XJGR12013071)
作者简介
Corresponding author. Tel： ＋86-993-2025167; E-mail： lily7588@163.com
