摘要
【目的】研究大鼠急性心肌缺血后不同时间再灌注心脏不同区域HSP70和Fos蛋白表达的规律 ,为心肌缺血 /再灌注损伤所致心性猝死的法医学诊断提供形态学依据。【方法】结扎大鼠左冠状动脉前降支建立急性心肌缺血 /再灌注模型。用免疫组织化学方法 ,观察心肌缺血后不同时间再灌注HSP70和Fos蛋白在心肌组织表达的改变。【结果】对照组心肌组织无Fos和HSP70表达。心肌缺血 15min再灌注 0 5h后Fos蛋白即在缺血区域表达 ,随着再灌注时间延长表达增强 ;非缺血区域 1h始有表达 ,缺血区域表达明显强于非缺血区域。心肌缺血区域再灌注 1h后HSP70始有表达 ,随着再灌注时间延长表达增强 ;非缺血区域心肌 2h始有表达 ,且明显弱于缺血区域。再灌注早期HSP70和Fos首先在心肌内层表达 ,随着时间延长表达向心肌外层扩展。【结论】心肌缺血 /再灌注早期不同时间HSP70和Fos蛋白表达有不同的区域性及强度 ,有可能为早期心肌缺血
Objective To study the expressions of Fos protein and HSP70 in the early stage of acute myocardial ischemia/reperfusion(I/R) in rats , and search the objective morphologic evidence for the forensic practice. Methods I/R rat model was induced by ligation of the anterior branch of the left coronary artery. The expressions of Fos protein and HSP70 were detected with immunohistochemistry. Results There were no expressions of Fos protein and HSP70 in control group. Fos protein began to express 0 5 h after reperfusion following 15 min ischemia in the ischemic areas, 1 h in the non ischemic areas and more evidence with prolongation of reperfusion. The expression of HSP70 began to increase in myocytes 1 h after I/R in the ischemic areas, 2 h in the non ischemic areas and more evidence with prolongation of reperfusion. The expressions of both of Fos protein and HSP70 were weaker in non ischemic areas than in ischemic areas. Meanwhile the expressions of HSP70 and Fos protein appeared first in the inner layer of myocardium and extended toward the outer layer of myocardium. Conclusion I/R induced the expressions of HSP70 and Fos protein and the location and intensity changed in difference stages. These results may be beneficial to the diagnosis of the early stage of acute myocardial I/R.
出处
《中山医科大学学报》
CAS
CSCD
北大核心
2001年第2期116-118,共3页
Academic Journal of Sun Yat-sen University of Medical Sciences
基金
汕头大学医学院"2 11"工程重点学科建设资助课题 !(199913)
关键词
急性心肌缺血
再灌注损伤
HSP70
FOS蛋白
表达
法医学
myocardial reperfusion injury/pathology
heat shock proteins 70
proto oncogene proteins c fos
immunohistochemistry
forensic medicine
