摘要
目的:观察肝郁脾虚型溃疡性结肠炎(ulcerative colitis,UC)大鼠结肠组织中PPAR-γmRNA及蛋白表达水平的动态变化,探讨其在肝郁脾虚型UC发病中的作用机制。方法:将50只Wistar大鼠随机分为5组:空白组、模型3天组、模型7天组、模型14天组、模型21天组,每组10只,采用TNBS/乙醇灌肠+束缚法+饮食失节法建立肝郁脾虚型UC大鼠模型,肉眼观察结肠组织大体形态并评分,免疫组化S-P法和RT-PCR法检测各组大鼠结肠组织中PPAR-γ基因和蛋白表达。结果:建模后3天大鼠结肠出现明显炎症和溃疡,且随着时间推移而加重,7天组结肠溃疡和炎症最为严重,21天组溃疡和炎症已经有所修复。不同时间段各模型组大鼠结肠组织中PPAR-γmRNA和蛋白的表达与空白组比较均显著降低(P<0.01),以7天组降低最为明显,14天和21天组的表达量逐渐升高,7天、14天与21天组之间比较,差异有显著性意义(P<0.01,P<0.05)。结论:肝郁脾虚型UC的发生发展与PPAR-γ的表达受抑密切相关,上调PPAR-γ的表达有望成为肝郁脾虚型UC治疗新靶向。
Objective：To study the dynamic expression of PPAR -γin rats＆#39;serum with ulcerative colitis （ UC） of hepatic stagnation and spleen deficiency type and identify its underlying mechanism of action .Methods：60 rats were randomly divided into five groups：the blank group,the model 3 day group,the model 7 day group,the model 14 day group and the model 21 day group,and every group had 12 rats.Prepare the UC models by means of the TNBS/ethanol enema and binding method and intemperance of taking food .Macroscopic observation in morphology , then give it the score .Detect PPAR-γgene and protein expressions in rats＆#39;serum by RT-PCR and immunohistochemical method .Results： Three days later , inflammation and ulceration occurred , in the 7-14 days, inflammation and ulceration were the most serious . In the 21 day of the inflammation and ulceration had been repaired .Compared with the blank group and ethanol group , in the each time point , the PPAR-γgene and protein expressions were decreased significantly （ P〈0 .01 ） , especially the 7 day .The expressions of 14 day and 21 day were gradually increased .Compared with these three model groups the discrepancy was statistically significant （P〈0.01,P〈0.05）.Conclusion：The occurrence and development of the UC of hepatic stagnation and spleen deficiency type are closely related to the PPAR -γ’ s inhibition of expression , upregulating the expression of PPAR-γis expected to become a new target for the treatment of UC of hepatic stagnation and spleen deficiency type .
出处
《中医药学报》
CAS
2014年第3期10-13,共4页
Acta Chinese Medicine and Pharmacology
基金
国家自然科学基金(No.81060283)
甘肃省自然科学基金(1107RJZA222)
关键词
肝郁脾虚
溃疡性结肠炎
PPAR-Γ
动态表达
Hepatic stagnation and spleen deficiency type
Ulcerative colitis
PPAR-γ
Dynamic expression
作者简介
曹燕飞（1987－），女，硕士研究生。研究方向：治则治法理论及其应用研究
通讯作者：朱向东（1973－），男，副教授，硕士研究生导师，主要从事中医药防治溃疡性结肠炎的应用基础研究。
