摘要
对北京某种羊场的疑似绵羊肺腺瘤病的羊进行临床诊断、病理组织学观察和PCR检测。结果显示,(1)"小推车试验"时从鼻腔里流出黏稠鼻液;(2)剖检可见肺脏肿大和实变,肺脏和气管内有大量泡沫性液体;(3)病理组织观察到肺脏上皮细胞肿瘤性增生,突入肺泡腔;(4)PCR和半巢式PCR能扩增到176bp和129bp的特异性片段;(5)序列比对分析显示,U3区与外源性绵羊肺腺瘤病毒的核苷酸同源性为90.9%-97.7%。综合上述诊断结果确诊该种羊场的疑似病例为绵羊肺腺瘤病。
In order to diagnose the suspected cases of ovine pulmonary adenomatosis （OPA） in Beijing, the sheep were studied by clinical diagnosis, histopathological observation and PCR assay. The results showed that： （1） Large Volumes of mucus exudates poured from their nose upon＂wheel-barrow test＂; （2）Necropsy showed lung enlargement and consolidation, a lot of foamy liquid was found in lung and trachea; （3）The pathological observation on the lung tissue showed neoplastic proliferation of alveolar epithelial cells were, and their influx into alveolar spaces; （4）The results of PCR and hemi-PCR showed that the amplified fragment lengths were 176 bp and 129 bp, respectively; （5）Sequence alignment analysis demonstrated that it had 90.9%-97.7% nucleotide acid ho- mology to that of exogenous jaagsiekte sheep retrovirus（exJSRV）. So the sheep were diagnosed OPA.
出处
《中国兽医杂志》
CAS
北大核心
2014年第5期22-24,共3页
Chinese Journal of Veterinary Medicine
关键词
绵羊肺腺瘤病
绵羊肺腺瘤病毒
病理组织学
PCR诊断
ovine pulmonary adenomatosis
jaagsiekte sheep retrovirus
histopathological observation
PCR assay
作者简介
王林（1983-），男，兽医师，博士，主要从事动物分子病毒学研究，E-mail：wang0631@163．com
通讯作者：冯小宇，E-mail：hairen-2000@sina．com
