摘要
参考禽流感病毒(AIV)基质蛋白(M)基因设计了两对引物,特异性识别其6个不同位点,建立了基于颜色判定的环介导等温扩增反应(LAMP)方法。试验对不同禽源病毒进行了测试,结果发现该方法仅特异性地检出禽流感病毒(H9和H5亚型)核酸,对AIV病毒的检测极限为10 EID50。人工感染病料检测结果与鸡胚病毒分离法的符合率为96.7%。数据表明,建立的LAMP方法特异、敏感、简便,在禽流感的诊断中具有很大的临床应用潜力。
With reference to the matrix protein gene of Avian Influenza Virus（ AIV）,we designed two pairs of primers to specifically recognize its six different sites,and established the Loop-mediated Isothermal Amplification Reaction（ LAMP） method based on the determined color. With different avian viruses tested,the results showed that the method only specifically detected AIV（ H9 and H5 subtype） nucleic acids,and the detection limit for AIV was10 EID50. We also detected the samples for artificial infections and discovered that the coincidence rate of LAMP to the virus isolation was 96. 7%. The experimental data demonstrated that the established methods were specific,sensitive,and simple,which had great potential for clinical application.
出处
《浙江农业学报》
CSCD
北大核心
2014年第3期576-580,共5页
Acta Agriculturae Zhejiangensis
基金
"山东省现代农业产业技术体系项目资助"
质检公益性行业科研专项(201010067)
关键词
禽流感病毒
基质蛋白基因
环介导等温扩增反应
avian influenza virus
matrix protein gene
loop-mediated isothermal amplification
作者简介
王辰雨（1988-），男，山东省济南市人，硕士研究生，研究方向为动物传染病。E—mail：milan8804@163．com
通讯作者，宋敏训，E-mail：mxsong@aliyun．com；
通讯作者，张鹤晓，E-mail：aqlzhx@126．com
