摘要
目的研究附子不同组分单次给药致小鼠神经毒性的时毒关系。方法取小鼠按不同时间点或不同组分分组，分别给小鼠灌胃附子不同组分，观察小鼠死亡情况和毒性反应，分别于药后相应时间点观察记录小鼠自主活动数和转棒数，检测血清过氧化物酶（SOD）、丙二醇（MDA）、乳酸脱氢酶（LDH）活性以及神经元特异性烯醇化酶（NSE）含量变化。结果水提组分组小鼠自主活动先加强后减弱再加强，血LDH药后60min升高显著；醇提组分组小鼠自主活动先减弱后加强，转棒停留时间先减少或增多，60min降低显著，血SOD含量在15～120min内持续降低；全组分组小鼠自主活动和转棒停留时间均在90min减少显著，血SOD含量减低，但显著性差异小于醇捉组分。3种组分组小鼠血清的神经元特异性烯醇化酶（NSE）含量均有不同程度的升高，且醇提〉水提〉全组分。结论单次给小鼠灌胃-定剂量的附子不同组分可造成一定的神经损伤，且醇提组分的损伤程度最大，并呈现-定的神经毒性“时-毒”关系。对损伤机制和进一步损伤所导致的行为学、递质水平、神经元等留待后续研究。
Objective To study the time-toxicity relationship caused by different components of Radix Aconite Lat-eralis to mice. Methods Mice were grouped according to different time, to observe the death condition and toxicity ofmice. The numbers of autononfic activities and rotarod test were observed and recorded. The changes of the activity ofSOD, MDA, LDI--I and NSE were detected. Results The changes of autonomic activities of mice with waterextraction were strengthened and then weakened, the changes of LDH in serum were lifted marked at 30 minutes.The changes of autonomic activities and standing time of mice with alcohol extraction were weakened and thenstrengthened, significant reduction was at 60 minutes, the changes of SOD in serum were reducing marked from 15to 120 minutes. The changes of autonomic activities and standing time of mice with whole components were reducedat 90 minutes, the changes of SOD in serum were reducing. The levels of neuron specific enolization enzyme（NSE）from the three groups＇ mice serum were changed to rise in different degree, and alcohol extraction 〉 water extraction〉 the whole components. Conclusion Single intragastric administrations of different components of Radix AconiteLateralis with certain dosage may induce acute nerve injury in mice and show certain time-toxicity relationship.The study on damage mechanism and the change of behavioral science, neurotransmitter levels and neuron wouldbe discussed in the following study.
出处
《中国药物警戒》
2014年第6期321-324,共4页
Chinese Journal of Pharmacovigilance
基金
国家重点基础研究发展计划(973)中医基础理论专项资助项目(2009CB522802)
关键词
附子
不同组分
神经毒性
“时-毒”关系
Radix Aconite Laterahs
different components
neurotoxicity
time-toxicity relationship
作者简介
王懿，女，在读硕士，中药药理与毒理。
通讯作者：孙蓉，博士，研究员·博导，中药药理与毒理。E-mail：sunrong107@163．com
