摘要
为了提高辐照处理芒草木质纤维素的酶解糖化效果,选用经过400 kGy60Coγ-射线辐照处理的芒草样品,考察在不同NaOH浓度、时间以及温度下处理对辐照芒草酶解产还原糖量的影响,得出最佳NaOH处理条件组合。正交试验结果表明,使用1.5%浓度的NaOH,在100℃条件下,水浴4 h的辐照芒草样品,经酶解还原糖含量高达452.49 mg/g。葡萄糖和木糖分别由纤维素和半纤维素定向转化而来,二者回收率依次为92.59%和75.98%。
In order to improve enzyme decomposition efficiency of lignocellulose from Chinese silvergrass treated by irradiation, this experiment selected Chinese silvergrass of 400 kGy 60Co γ-ray irradiation, investigated the yield of reducing sugars in different NaOH concentration, time and temperature by enzyme treatment, then obtained the best optimum conditions. The results showed that the concentration of 1.5%NaOH at 100℃ for 4 h, the reducing sugar production was high to 452.49 mg/g. Glucose and xylose were respectively converted from cellulose and hemicellulose, their rate of recovery were run up to 92.59% and 75.98%.
出处
《中国农学通报》
CSCD
2014年第14期185-191,共7页
Chinese Agricultural Science Bulletin
基金
国家863计划课题"基于射线辐照和嗜热真菌热稳定纤维素酶的纤维素降解新技术"(2012AA101804)
关键词
辐照
芒草
NaOH处理
酶解
木质纤维素
irradiation
Chinese silvergrass
NaOH treatment
enzyme decomposition
lignocellulose
作者简介
第一作者简介:彭姿,女,1989年出生,湖南娄底人,硕士,研究方向为农产品加工与贮藏专业。通信地址:410128湖南农业大学食品科技学院,E—mail:42339918@qq.com。
通讯作者:谭兴和,男,1959年出生,教授,博士,研究方向为农产品加工研究。通信地址:410128湖南农业大学食品科技学院,Tel:0731—84673524,E—mail:xinghetan@163.com。