摘要
目的:研究羟喜树碱对人肾上腺皮质癌SW-13细胞生长抑制和凋亡作用的影响。方法:采用CCK8检测不同浓度羟喜树碱对SW-13细胞增殖的影响;流式细胞仪测定药物作用前后的细胞凋亡情况。结果:不同浓度的羟喜树碱分别作用24,48,72h后,SW-13细胞的生长增殖均受到明显抑制(P<0.01),并呈时间和浓度依赖性;流式细胞术显示SW-13细胞凋亡率随羟喜树碱浓度的增加而升高。结论:羟喜树碱可以显著抑制人肾上腺皮质癌SW-13细胞生长,促进其凋亡。
Objective：To investigate the effect of hydroxycamptothecin （HCPT）,a antineoplasticon,on the proliferation and apoptosis of human adrenal cortex carcinoma cell line SW-13.Methods：The cell-growth inhibition of HCPT on SW-13 cells was assessed by CCK8 assay.The cell apoptosis was measured by flow cytometry when treated with HCPT.Results：HCPT significantly inhibited the SW-13 cell proliferation （P〈0.01） with a time-and concentration-dependent manner.Flow cytometry displayed that the apoptosis rate of SW-13 cell increased with HCPT concentration increase.Conclusion：The results showed that HCPT not only can significantly inhibit the growth of adrenal cortical carcinoma SW 13 cells,but also promote apoptosis.
出处
《广西医科大学学报》
CAS
2014年第2期173-176,共4页
Journal of Guangxi Medical University
基金
广西自然科学基金青年基金资助项目(2010GXNSFB013062)
国家自然科学基金资助项目(No.81060220)
广西卫生厅自筹经费科研课题(No.Z2013037)
作者简介
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