摘要
目的繁殖及鉴定NLRP3基因敲除(NLRP3-/-)小鼠。方法将引进的NLRP3基因敲除杂合子小鼠,单独进行饲养及配种繁殖,繁殖后其子代出现3种基因型:野生型、杂合子型及纯合子型,提取每只小鼠的基因组DNA,用PCR和T7E1酶切方法进行鉴定,将获得的纯合子小鼠与异性杂合子交配,以获得更多的基因敲除纯合子小鼠。结果 NLRP3杂合子小鼠的饲养及繁殖均获得成功,获得了NLRP3基因敲除纯合子和杂合子小鼠。结论正确的饲养、繁殖及鉴定方法是从NLRP3基因敲除杂合子小鼠中获得纯合子小鼠的有效途径。
Objective To breed and identify NLRP3 gene knock-out mice. Methods The NLRP3 gene knock-out heterozygote mice were bred alone and copulated. The offsprings were to have three genotypes., wild genotype, heterozygote genotype and homozygote genotype. Genomic DNA was obtained from each pups and were subjected to PCR and T7 endonuclease 1 to identify the genotype. The homozygote mice were mated with the opposite sex heterozygote mice to obtain more homozygote pups. Results Breeding and reproducing were both successful, and we obtained heterozygote genotype and homozygote genotype mice with NLRP3 gene knock-out. Conclusion Correct methods of breeding, reproducing and identifying can effectively obtain NLRP3 gene knock-out mice from heterozygote mice.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2014年第5期555-559,共5页
Academic Journal of Second Military Medical University
基金
国家"重大新药创制"科技专项(2014ZX09J14106)
海市公共卫生重点学科建设计划项目(12GWZX0501)
上海市自然科学基金(13ZR1449400)~~
作者简介
冯世杰，硕士．E—mail：fxiaojie1120@126．com
通信作者（corresponding author）．Tel：021—81871032，E—mail：newdrug@smmu．edu．cn
