摘要
金黄色葡萄球菌是奶牛乳房炎的最主要病原之一,由其引起的奶牛乳房炎发病率高、防治困难,常常会给奶业造成巨大经济损失,对食品安全造成巨大威胁。以新疆阿克苏地区奶牛乳房炎性金黄色葡萄球菌分离株为研究对象,详细观察了菌株在刚果红培养基上的形态,并通过K-B扩散法分析了5株金黄色葡萄球菌乳房炎分离株对19种抗生素的耐药情况。结果表明,5株金黄色葡萄球菌均具有生物被膜形成能力,且对青霉素、链霉素、氨苄西林和四环素等常用抗生素完全耐药,对万古霉素、环丙沙星、诺氟沙星、阿米卡星等抗生素中度敏感,而对恩诺沙星、头孢西丁和头孢噻肟3种抗生素高度敏感。研究结果提示,乳房炎性金黄色葡萄球菌具有较强的耐药性,而这种耐药性可能与其生物被膜形成有关。
Staphylococcus aureus is one of main pathogens that causes the mastitis of dairy cow. Because the mastitis of dairy cow had higher incidence and it was difficult to be cured, so it brought great economic loss for the milk industry and had greater threatens for the food safety. Taking the isolated strains of mastitis-derived S. aureus as the research objects, their morphology on Congo red medium was observed. The drug sensitivity of 5 isolated strains of mastitis-derived S. aureus to 19 kinds of antibiotics was analyzed by using diffusion method. The results showed that 5 strains of S. aureus all had the ability of forming the biofilm. They were totally tolerant to common antibiotics of penicillin, streptomycin, ampicillin and achromycin, and they were moderately sensitive to vancomycin, ciprofloxacin, norfloxacin, amikacin and other antibiotics. The isolated strains were highly sensitive to 3 kinds of antibiotics （enrofloxacin, cefoxitin and cefotaxime）. The research results indicated that mastitis-derived S. aureus had stronger drug tolerance, and the drug tolerance might be related with the forming of its biofilm.
出处
《畜牧与饲料科学》
2014年第4期3-6,共4页
Animal Husbandry and Feed Science
基金
塔里木大学大学生创新创业训练项目(2013036
2013037)
关键词
金黄色葡萄球菌
奶牛乳房炎
菌落形态
耐药性
生物被膜
Staphylococcus aure us
mastitis of dairy cow
colony morphology
drug tolerance
biofilm
作者简介
杨晓飞（1992-），男，所学专业为动植物检疫。
通讯作者：贺建忠（1977-），男，副教授，硕士，主要研究方向为临床兽医学。
