摘要
本试验旨在研究转基因豆粕对崂山奶山羊生长性能、肌肉营养成分及组织器官中外源基因转移的影响。选取30只50日龄崂山奶山羊随机分为2组,每组3个重复,每个重复5只。精饲料分别以非转基因豆粕和转基因豆粕(MON89788和GTS40-3-2)配制,粗饲料均为干草。试验期150 d。结果表明:2组奶山羊平均日采食量、平均日增重和料重比均无显著差异(P>0.05);奶山羊肌肉的干物质、粗脂肪、粗蛋白质、粗灰分、钙、磷含量2组间亦均无显著差异(P>0.05);采用实时定量PCR法检测奶山羊肌肉、肝脏、胰腺、肾脏、脾脏、胸腺、肺、心脏、皱胃、小肠组织的外源基因,均未检测出转基因片段。可见,转基因豆粕(MON89788和GTS40-3-2)对崂山奶山羊生长性能和肌肉品质均无显著影响,转基因豆粕的外源基因在奶山羊肌肉及各组织器官未发生水平转移。
Abstract： In order to analyze the effects of genetically modified soybean meal on growth pertormance, meat nutrient composition, and exogenous gene transformation to tissues and organs of Laoshan dairy goats, thirty 50-day-old Laoshan dairy goats were randomly divided into two groups with three replicates in each group and five goats per replicate. Goats in different groups were fed concentrates containing non-genetically modified soybean meal and genetically modified soybean meal （MON89788 and GTS40-3-2 ）, respectively, and were fed the same roughage （ Chinese wildrye）. The experiment lasted for 150 days. The results showed as fol- lows： average daily feed intake, average daily gain and feed to gain ratio in the two groups were not signifi- cantly different （P 〉0.05） ; the contents of dry matter, ether extract, crude protein, ash, calcium and phos- phorus in muscle of dairy goats were not significantly different either （ P 〉 0.05）. Moreover, no gene rag- ments of genetically modified soybean meal were detected from muscle, liver, pancreas, kidney, spleen, thy- mus, lung, heart, abomasum and small intestine of dairy goats by real-time quantification PCR. The results suggest that genetically modified soybean meal （MON89788 and GTS40-3-2 ） had no significant effects on growth performance and muscle quality of Laoshan dairy goats, and no genetic horizontal transformation is ob- served in tissues and organs.
出处
《动物营养学报》
CAS
CSCD
北大核心
2014年第4期1028-1033,共6页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
动物卫生风险分析关键技术与应用研究(200903055)
关键词
转基因豆粕
奶山羊
生长性能
肌肉品质
genetically modified soybean meal
dairy goat
growth performance
meat quality
作者简介
刘斌（1989-），男，山东威海人，硕士研究生，研究方向为动物营养与饲料科学。E-mail：282755781@qq.com
通讯作者：王述柏，教授，硕士生导师，E-mail：wshubai@163．com
