摘要
目的探讨脂肪来源的间充质干细胞(ADSCs)对大鼠急性心肌梗死(AMI)后心功能的影响。方法结扎SD大鼠左冠状动脉前降支制备心肌梗死模型。同种异体ADSCs体外分离、培养、纯化、扩增,在大鼠AMI区域周围进行心外膜下移植。将24只大鼠随机分为3组,每组8只:A组为AMI模型组,只给予前降支结扎;B组为细胞培养基(DMEM)移植组,结扎后心外膜注射DMEM;C组为ADSCs治疗组,结扎前降支后接受ADSCs移植治疗。术后7 d、28 d各组大鼠行心脏超声检测左室射血分数(LVEF)、左室短轴缩短率(FS),28 d超声检测后行血流动力学测量左室收缩压、左室舒张末压、左心室压力最大变化速率,评价ADSCs移植对AMI后大鼠心功能的影响;心脏组织行TTC染色,观察AMI面积。结果与AMI模型组比较,ADSCs治疗组的LVEF、FS及血流动力学指标明显提高(P<0.01)。TTC染色观察心肌梗死面积,ADSCs治疗组心肌梗死面积明显减小(P<0.01)。结论 ADSCs可减少AMI的面积,改善心功能。
Objective To investigate the effects of adipose-derived mesenchymal stem cells （ADSCs） transplanted on cardiac function after acute myocardial infarction in rats. Methods The model of acute myocardial infarction （AMI） was created by occluding the left anterior descending artery （LAD） in SD rats. Allogeneic ADSCs were isolated, purified, amplifed and conducted epicardial trans- plantation. The rats were randomly divided into 3 groups with 8 rats for each group. Group A was the model of AMI. Group B （control group） was injected DMEM. Group C received ADSCs treatment. After 1 and 4th week, cardiac ultrasound was inspected. After the in- spection of cardiac ultrasound at 4th week, index of hemodynamies were tested to evaluate the cardiac function. The area of myocardial in- farction wre calculated with Trc staining. Results Compared with AMI group, left ventricular ejection fraction, fractional shortening and index of hemodynamics of ADSCs group were significantly increased （ P 〈0.01 ）, and the myocardial infarction area of ADSCs group was significantly lower （ P 〈0.01 ）. Conclusion ADSCs can reduce area of myocardial infarction and improve the function of heart.
出处
《中国体外循环杂志》
2014年第1期54-57,共4页
Chinese Journal of Extracorporeal Circulation
基金
国家高技术研究发展计划(2011AA020101)
关键词
脂肪间充质干细胞
急性心肌梗死
心功能
大鼠
Adipose-derived stem cells
Acute myocardial infarction
Cardiac function
Rat
作者简介
通讯作者：陈光辉，Email：chenkevin301@msn．com
