摘要
目的:应用转化生长因子-β1(TGF-β1)诱导肌管转分化,研究肝细胞生长因子(HGF)对肌管表型重塑的作用及机制。方法:采用C2C12细胞系诱导分化形成肌管,细胞随机分为3组,空白对照组无生长因子干预,诱导组培养液中加入TGF-β1(5 ng/mL),抑制组培养液中同时加入TGF-β1(5 ng/mL)和HGF(30 ng/mL)。培养12 h后,利用Western blot法检测结缔组织生长因子(CTGF)蛋白表达,用实时荧光定量PCR法检测CTGF mRNA表达。结果 :诱导组CTGF的蛋白、mRNA表达水平均较空白对照组增加,抑制组CTGF的蛋白、mRNA表达水平均较诱导组降低(P<0.05)。结论:HGF可以抑制TGF-β1对肌管表达CTGF的作用,为研究骨骼肌细胞转分化提供了依据。
Objective To study the influence and mechanism of hepatocyte growth factor （HGF） on myotube phenotype by myotube transdifferentiation induced by transforming growth factor-β1 （TGF-β1）. Methods C2C12 cells were cultured in differentiation medium to induce myotubes formation. The cells were randomly derided into 3 groups. The control group without growth factor interruption. The induction group was supplemented with TGF-β1 （5 ng/mL） while the inhibition group was supplenmented with both TGF-β1 （5 ng/mL） and HGF （30 ng/mL）. After 12 hours, the expressions of connective tissue growth factor （CTGF） protein in myotubes were detected by Western blot, the levels of CTGF mRNA were measured by RT-PCR. Results Compared to the control group, the protein and mRNA levels of CTGF significantly increased in TGF-β1 treated group, whereas the protein and mRNA levels of CTGF were significantly lower in inhibition group than those in induction group （P 〈 0.05）. Conclusion HGF can inhibit the effect of TGF-β1 on the expression of CTGF in myotubes, which provides the evidences on the study of skeletal muscle cell transdifferentiation.
出处
《实用医学杂志》
CAS
北大核心
2014年第7期1045-1047,共3页
The Journal of Practical Medicine
基金
国家自然科学基金面上项目(编号:81070774)
南京军区面上项目A类(编号:10MA024)
作者简介
通信作者：郑宏良E-mail：zhenghl_2004@163．com
