摘要
目的了解综合医院腹泻患者非伤寒沙门菌感染情况,分析非伤寒沙门菌的血清分型、耐药性和分子特征。方法对2009年9月-2011年6月腹泻病患者送检的767份粪便标本进行非伤寒沙门菌检测,对分离到的菌株进行血清分型、药物敏感性试验和脉冲场凝胶电泳(PFGE)分型。结果从767份腹泻粪便标本中分离到36株非伤寒沙门菌,阳性检出率为4.56%;以儿童为主,占65.71%;共分为10种血清型,主要以鼠伤寒沙门菌和肠炎沙门菌为主,分别占37.14%和28.57%;10个血清型非伤寒沙门菌对头孢类和环丙沙星的敏感率>84.00%,除斯坦利沙门菌和山夫登宝沙门菌外,其他血清型均对多种抗菌药物产生不同程度的耐药或交叉耐药;将13株鼠伤寒沙门菌和10株肠炎沙门菌共分离出19个PFGE分型,其中2株肠炎沙门菌PFGE同型,3株鼠伤寒沙门菌PFGE同型,存在不同耐药谱。结论综合医院引起感染性腹泻的非伤寒沙门菌主要为鼠伤寒沙门菌和肠炎沙门菌,儿童多见;非伤寒沙门菌多药耐药严重,临床在应用氟喹诺酮类治疗非肠外沙门菌属感染时,应根据药敏结果慎重选择。
OBJECTIVE To understand the incidence of non-typhoid Salmonell infections in the diarrhea patients from a general hospital and then analyze the serotypes, drug resistance as well as the molecular characteristics of the non-typhoidal Salmonella strains. METHODS Totally 767 feces specimens obtained from the diarrhea patients from Sep 2009 to Jun 2011 were detected for the no n-typhoidal Salmonella strains, then the serotyping, drug sus- ceptibility testing, and pulsed field gel electrophoresis （PFGE） assay were performed for the isolated strains. RESULTS A total of 36 strains of non-typhoidal Salmonella were isolated from 767 copies of feces specimens with the positive detection rate of 4.56%, among which 65.71% were isolated from the children. Of totally 10 sero- types detected, Salmonella typhimurium （37. 14%） and Salmonella enteritidis （28. 57%） were dominant. The drug susceptibility rates of the non-typhoidal Salmonella to cephalosporins and ciprofloxacin were more than 84.00 % ; all the serotypes, except for Salmonella stanley and Salmonella senftenberg, varied in the drug resist- ance or cross drug resistance to multiple antibiotics. Totally 19 PFGE types were detected from 13 strains of Sal- monella typhimurium and 10 strains of Salmonella enteritidis, among which 2 strains of Salmonella enteritidis had the same PFGE type, and 3 strains of Salmonella typhimurium had the same PFGE type, varying in the drug resistance spectrum. CONCLUSION Salmonella typhimurium and Salmonella enteritidis are the predominant non typhoid salmonell causing infectious diarrhea, which is common in children; the non-typhoidal Salmonella strains are highly multidrug-resistant, as the fluoroquinolones are used for the treatment of non-parenteral salmonella infections, it is necessary to reasonably use antibiotics based on the result of drug susceptibility testing.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2014年第8期1874-1876,共3页
Chinese Journal of Nosocomiology
基金
中美新发和再发传染病合作基金项目(IU2GGH000018-01)
关键词
腹泻
非伤寒沙门菌
监测
病原学特征
Diarrhea
Non-typhoid salmonell
Monitoring
Etiological characteristic
