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NOD1和NOD2基因在牦牛雄性生殖器官中的表达检测

Expression Profile of Bos grunniens NOD1 and NOD2 Gene in Tissues of Male Reproductive System
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摘要 为了解NOD1和NOD2基因在牦牛生殖系统中的表达情况,从脾脏组织总RNA中RT-PCR扩增牦牛NOD1和NOD2基因,半定量RT-PCR分析NOD1和NOD2 mRNA在牦牛雄性生殖组织的表达水平。结果显示,NOD1在脾脏、睾丸、附睾头、附睾体、附睾尾、输精管和阴茎中均相对低表达,而NOD2在脾脏、睾丸、附睾头、附睾体、附睾尾、输精管和阴茎中均相对高表达。结果表明,NOD1和NOD2在牦牛雄性生殖组织中广泛表达,提示其在牦牛生殖过程中可能发挥一定的作用。 The purpose of the present study was to investigate expression of NOD1 and NOD2 genes in reproductive system of male yak (Bos grunniens). Semi-quantitation RT-PCR was used for analyzing the expression profiles of the NOD1 and NOD2 mRNA. The results showed that the expression level of NOD1 was relatively low in spleen, testis, epididymis, spermaduct and penis, while NOD2 was abundant in these tissues. These results suggested that NOD1 and NOD2 genes may play an important role in reproduction of male yak.
出处 《四川动物》 CSCD 北大核心 2014年第2期261-264,共4页 Sichuan Journal of Zoology
基金 四川省教育厅青年基金项目(09zb054) 教育部长江学者和创新团队发展计划创新团队项目(IRT0848) 国家自然科学基金项目(31172379)
关键词 牦牛 NOD1基因 NOD2基因 组织表达 Bos grunniens NOD1 gene NOD1 gene tissue expression
作者简介 余树民(1968~),男,副教授,博士,从事兽医产科学研究,E-mail:yayushumin@163.com 通讯作者Corresponding author,E-mail:suizhongcao@126.com
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参考文献13

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二级参考文献44

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