摘要
目的观察体外受精-胚胎移植(IVF-ET)治疗周期中未见原核(PN)晚期卵裂胚胎单个卵裂球染色体分子核型。方法取IVF-ET治疗中4枚未见PN晚期卵裂胚胎进行体外培养,在受精第3天行胚胎活检,对单个卵裂球行微阵列-比较基因组杂交(microarray-CGH)染色体分析。结果 4枚未见PN受精卵染色体均发生非整倍体变异。其中1枚胚胎性染色体为X染色体单体,3枚胚胎性染色体为XY正常二倍体。但4枚胚胎均存在常染色体变异。结论未见PN晚期卵裂胚胎染色体microarray CGH分析结果表明,其单个卵裂球全基因组呈非整倍性变异,未见PN晚期卵裂胚胎存在常染色体和性染色体变异风险。
Objective To analyze the aneuploidy of single blastomeres from nonpronuclear embryos on cleavage stage in in vitro fertilization and embryo transfer （IVF-ET） cycle by microarray comparative genomic hybridization （microarray-CGH）. Methods The four non-pronuclei embryo were achieved in one IVF cycle, and microarray-CGH was applied to analyze the aneuploidy of single cells biopsied from the four cleavage-stage embryos on day 3 after insemination. Results The four blastomere had non euploid variation in 24 chromosomes. Out of them, one had X monomer in sex chromosome, and the other three had normal XY diploid in sex chromosome. The four embryos had autosomal mutations, such as gene deletion. Conclusions The aneuploidy analysis by microarray-CGH shows that the blastomeres from nonpronuclear embryos on cleavage-stage in IVF cycle are aneuploid, and nonpronuclear embryos have the risk of abnormal sex chromosome and autosome chromosomes.
出处
《中华妇幼临床医学杂志（电子版）》
CAS
2013年第6期653-656,共4页
Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)
关键词
体外受精-胚胎移植
微阵列-比较基因组杂交
无原核
卵裂球
in vitro fertilization and embryo transfer
microarray comparative genomichybridization
non-pronuclei
blastomere
作者简介
通信作者：张守信，Email：9712zhangsx@163．com
