摘要
纳米抗体作为一种新型抗体片段，保留了完整的抗原结合部位，具有相对分子质量小、特异性高、亲和力强等特性，非常适于构建分子探针用于分子影像。体外实验显示，以纳米抗体为基础的分子影像探针与抗原的亲和力可达到nmol水平。在肿瘤、动脉粥样硬化、神经系统疾病等的动物模型中，纳米抗体分子探针可高特异性地结合到靶器官，未结合的部分由肾脏迅速排泄，具有较高的靶／血液（T／B）比值，可清晰显示病变组织。经结构改造或化学修饰后，纳米抗体与靶器官的亲和力和滞留量进一步提高，更有利于其在分子影像中的应用。随着相关研究的不断深入，以纳米抗体为基础的分子影像在疾病早期诊断及疗效评估中的良好应用前景日渐突显。
The nanobody is a novel antibody fragment, which has beneficial biophysical and phar- macokinetic properties, such as the small molecular weight, high affinity and specificity for antigen. Nanobody is ideally suitable for molecular imaging as a targeting probe that could label antigen at nmol level in vitro. In animal models of xenografted tumor, atherosclerotie plaques and brain disorders, the target tissues were specifically and clearly detected and the high tumor-to-blood （T/B） ratios were obtained. Stiuctural or chemical modified nanobodies will have higher affinity and retention to target tissues, and be convenient for the application of molecular imaging. With the development of the related research, nanobody-based molecu- lar imaging will be gradually transformed into the clinical applications, and play an important role in early diagnosis and therapeutic assessment.
出处
《中华核医学与分子影像杂志》
CSCD
北大核心
2014年第1期68-72,共5页
Chinese Journal of Nuclear Medicine and Molecular Imaging
基金
国家自然科学基金（81071200,30830041,30970853）
湖北省自然科学基金杰出青年基金（2010CDA094）
湖北省分子影像重点实验室开放基金（02-03-2011-75）
关键词
纳米技术
抗体
分子显像
发展趋势
Nanotechnology
Antibodies
Molecular imaging
Trends
作者简介
通信作者：兰晓莉，Email：hzslxl@163．com
