摘要
通过设计能扩增奥尔森派琴虫和北海派琴虫ITS序列的特异性引物,对我国东南沿海8个地区养殖牡蛎中常见的两种派琴虫相应基因进行扩增、克隆和测序,并进行同源性分析。国内不同地区奥尔森派琴虫ITS序列的同源性超过99.3%,与国外分离株的同源性也在98.8%以上;种间同源性分析发现,奥尔森派琴虫与海水派琴虫和P.honshuensis的同源性较高,分别为94.2%和95.0%,与P.qugwadi的同源性最低,仅62.9%。深圳和三亚分离的北海派琴虫ITS序列有18个碱基存在差异,其中ITS-1有3个碱基差异,ITS-2有15个碱基差异,而5.8S高度保守,没有碱基差异;北海派琴虫与其他种类之间的差异主要表现在ITS-1和ITS-2区域;同源性比较发现,北海派琴虫的同源性在100%-97.9%之间,其中深圳北海派琴虫的同源性较低,为97.9%。种间同源性分析表明,北海派琴虫与奥尔森派琴虫的同源性较高,为89.4%,其次为海水派琴虫和P.honshuensis,与P.qugwadi同源性最低,仅71.9%。
By desiging specific primers which can amplify ITS sequences of P. olseni and P beihaiensis, Crassostrea gigas coming form 8 different farming areas were detected, cloned, sequenced and homology analyzed. The results showed that the ITS sequence homology of P olseni was higher than 99.3% in different areas of China, and higher than 98.8% compared with foreign isolates. The interspecific homology analysis showed, P. olseni had a higher homology with P. marinus and P. honshuensis, were 94.2% and 95.0% respectively, and had the lowest homology with P. qugwadi, only 62.9%. The ITS sequences ofP beihaiensis isolated from Shenzhen and Sanya had 18 base differences, ITS-1 had 3, ITS-2 had 15, and 5.8s had no differences respectively. The differences of P beihaiensis and other Perkinsosis were showed in ITS-1 and ITS-2 mainly. P beihaiensis isolated from Shenzhen had lower homology, only 97.9%, since the range was 100%-97.9%. The interspecific homology analysis showed, P. beihaiensis had a higher homology with P olseni, was 89.4%, followed by P. marinus and P. honshuensis, and had the lowest homology with P qugwadi, only 71.9%.
出处
《检验检疫学刊》
2014年第1期62-67,共6页
Journal of Inspection and Quarantine
基金
福建省自然科学基金项目(2012J05065)
国家质检公益专项项目(201210055)
