摘要
应用一种新型高灵敏度光电倍增管作为传感器,设计一套用于检测小麦超弱发光的光子计数测量系统,实现对储藏小麦超弱发光的定量测量.适当安排LED阵列分布,设计适合延迟发光需要的照明系统,通过对白光LED光源辐射等效单光子能量和流明效率的计算,建立起光照度与光子数强度之间的联系,实现了光源照射强度与样品延迟发光强度之间的定量比较.对不同光照强度下小麦样品延迟发光的测试和双曲函数拟合,探讨了小麦超弱延迟发光双曲函数拟合参量的变化规律;发现不同照射强度下存在不同的饱和照射时间;小麦超弱延迟发光强度曲线符合双曲衰减规律,超弱延迟发光持续时间达数小时.通过对光照时间和数据采集时间的精确控制,以及在拟合函数中设置延迟时间参量因子,实现了延迟发光测试数据对应时间点的精确标定.
A high sensitive single photon counting system with a new photomultiplier tube as a sensor was developed to detect the ultra-weak biophoton emission from wheat, which fulfilled the absolute quantity detection of photon emitted from wheat in the storage condition. An illuminating system for the delayed bioluminescence was designed with a proper LED array. The relation of the illuminatingintensity and the intensity of photons was established through calculation of equivalent single photon energy and lumen efficiency for white LED, and the quantity comparison of illumination intensity and delayed bioluminescence intensity was realized. There existed different illuminating saturation time at different illumination. The accurate calibration of time for testing data of the delayed bioluminescence was fulfilled by accurate control of the illuminating time and data acquisition time, and also setting the delayed time parameter in fitting function. The ultra-weak delayed bioluminescence from wheat was detected and analyzed systematically, and the results show that the intensity curves of delayed luminescenc1e from wheat follows a hyperbolic attenuation trend, and the duration of the luminescence lasts for several hours. Subsequently, the hyperbolic function fitting of testing data was carried out and the changing regularity of fitting parameters was discussed.
出处
《光子学报》
EI
CAS
CSCD
北大核心
2014年第2期115-120,共6页
Acta Photonica Sinica
基金
国家自然科学基金(No.61078070)
河南省高校创新人才支持项目(No.2011HASTIT018)资助
关键词
超弱延迟发光
光子数强度
等效单光子
饱和照射时间
吸收比
Ultra-weak delayed bioluminescence
Photons intensity
Equivalent single photon
Illuminating saturation time
Absorption ratio
作者简介
第一作者：吴才搴（1968--），男，教授，博士，主要研究方向为光电检测技术．Email：wucaizhang@haut．edu．cn
