摘要
目的:研究复方氢溴酸东莨菪碱贴膏中氢溴酸东莨菪碱含量测定方法。方法:采用加热回流提取复方氢溴酸东莨菪碱贴膏中的氨溴酸东莨菪碱,并用高效液相色谱法测定其含量。色谱柱:Agilent ZORBAX SB-C_8柱(250 mm×4.6 mm,5μm),流动相:0.25%十二烷基硫酸钠溶液(用磷酸调节pH至2.5)-乙腈(60:40),检测波长:210 nm,流速:1.0 ml·min^(-1),进样量20μl。结果:氢溴酸东莨菪碱浓度在11.24~224.80μg·ml^(-1)范围内线性关系良好,r=0.999 9。平均加样回收率为98.22%,RSD为0.9%(n=6)。结论:所建含量测定方法简便准确,溶剂成本低,且较环保。
Objective： To study the determination of scopolamine hydrobromide in compound scopolamine hydrobromide adhesive plasters. Methods： Scopolamine hydrobromide was extracted from compound scopolamine hydrobromide plasters by heating reflux and the content was determined by HPLC. An Agilent ZORBAX SB-C8 （250 mm ×4.6 mm,5 μm） column was used with 0.25% SDS so- lution （ adjusting pH to 2.5 with phosphoric acid） -acetonitrile （ 60：40 ） as the mobile phase at a flow rate of 1.0 ml·min^-1 , the detec- tion wavelength was at 210nm and the injection volume was 20 μl. Results： Seopolam＇~ne hydrobromide had a good linear relationship within the concentration range of 11.24-224.80 μg·ml^-1 （ r = 0. 999 9 ）. The average recovery was 98.22% （ RSD = 0.9% , n = 6）. Conclusion： The heating reflux method is accurate and simple, and benefit for the environment protection.
出处
《中国药师》
CAS
2014年第1期170-172,共3页
China Pharmacist
作者简介
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