摘要
基于肝素钠对吖啶橙的共振散射增强作用，建立了一种测定肝素钠的新方法。在1．0mol／LHCl介质中，吖啶橙与肝素钠反应形成缔合物，使溶液共振散射（RS）增强，且在323nm波长处产生1个较强散射峰。在0．020～2．0μg／mL范围内，体系共振散射信号与肝素钠浓度之间有较好的线性关系，其检出限为0．185ng／mL。该方法灵敏、稳定、选择性好，可直接用于肝素钠注射液中肝素钠含量的测定，回收率为95．6％～102．99／6。
A new analytical method has been developed based on the enhancement of resonance scattering of heparin sodium to acridine orange. In 1.0 mol/L HC1 solutions, acridine orange reacted with heparin sodium to form ion-association complexs, which made the resonance scattering intensity enhance obviously and created the maximum resonance scattering peak at 323 nm. Under the optimum experimental conditions, there was a good linear relationship between the resonance scattering intensity of the system and the heparin sodium concentration in the range of 0. 020-2.0μg/mL, and the detection limit was 0. 185 ng/L. This method has been applied to determine the heparin sodium content in heparin sodium injection with the recovery in the range of 95.6%-102.9%.
出处
《分析科学学报》
CAS
CSCD
北大核心
2014年第1期111-114,共4页
Journal of Analytical Science
基金
四川省教育厅科研项目(2009ZC107)
关键词
肝素钠
吖啶橙
共振散射
Heparin sodium
Acridine orange
Resonance scattering
作者简介
通讯作者：翟好英，女，副教授，主要从事光谱分析研究．
